The role of gamma interferon in infection of susceptible mice with murine coronavirus, MHV-JHM.
The role of gamma interferon in infection of susceptible mice with murine coronavirus, MHV-JHM.
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γ干扰素在易感小鼠感染鼠冠状病毒 MHV-JHM 中的作用。
DOI：
10.1007/bf01316746
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发表时间：
1991
影响因子：
2.7
通讯作者：
Bottomly,K
中科院分区：
文献类型：
--
作者：
Smith,AL;Barthold,SW;deSouza,MS;Bottomly,K
用小鼠肝炎病毒JHM株（MHV-JHM）感染BALB/c小鼠，在与卵清蛋白（OVA）相关的几个时间间隔中，可导致OVA特异性IgG 2a滴度升高。由于γ干扰素（IFN）与IgG 2 a产生的上调有关，因此试图确定感染小鼠血清中这种细胞因子的水平是否被改变。血清中未检测到IFN-γ，但与模拟治疗的感染小鼠相比，用单克隆抗IFN-γ抗体治疗mhv - jhm感染小鼠可导致高死亡率、生存时间缩短、肝脏和脾脏病毒滴度增强以及更严重的病毒相关病理。重组IFN-γ免疫治疗可通过死亡率和靶器官病毒滴度改善疾病。
Infection of BALB/c mice with mouse hepatitis virus, strain JHM (MHV-JHM), at any of several intervals relative to ovalbumin (OVA) administration resulted in elevated OVA-specific IgG 2 a titers. Since gamma interferon (IFN) has been implicated as an up-regulator of IgG 2 a production, attempts were made to determine whether levels of this cytokine were modified in sera of infected mice. Serum IFN-γ was not detected, but treatment of MHV-JHM-infected mice with monoclonal anti-IFN-γ antibody resulted in high mortality with decreased survival times, enhanced virus titers in liver and spleen, and more severe virus-associated pathology, compared to mock-treated, infected mice. Immunotherapy with recombinant IFN-γ ameliorated disease as reflected by mortality rates and virus titers in target organs.