In vitro studies of the adenosine 3',5'-monophosphate (cAMP) response of embryonic rat calvaria to bovine parathyroid hormone-(1--84) [bPTH-(1--84)], BPTH-(1--34), and bPTH-(3--34) and the loss of cAMP responsiveness after prolonged incubation.
In vitro studies of the adenosine 3',5'-monophosphate (cAMP) response of embryonic rat calvaria to bovine parathyroid hormone-(1--84) [bPTH-(1--84)], BPTH-(1--34), and bPTH-(3--34) and the loss of cAMP responsiveness after prolonged incubation.
复制标题
胚胎大鼠颅盖腺苷 3,5-单磷酸 (cAMP) 对牛甲状旁腺激素-(1--84) [bPTH-(1--84)]、BPTH-(1--34)反应的体外研究
作者：
M. Herrmann‐Erlee;J. V. D. Van der Meer;J. Hekkelman
研究了牛甲状旁腺激素-（1-84）[bPTH-（l-84）]、bPTH-（l-34）和[Nle-8，Nle-18，Tyr-34]bPTH-（l-34）酰胺在8 × 10−8 M浓度下刺激胚胎大鼠颅骨中cAMP水平的时间过程。加入激素制剂后，cAMP水平在10分钟时达到最大值，4小时时下降至接近基础水平。将大鼠颅盖在含有相同浓度（8 × 10−8 M）bPTH-（1-84）的固体凝块上孵育2 h，重新加入激素后，反应性几乎完全丧失（cAMP水平没有显著进一步升高）。在用bPTH-（1-34）或[Nle-8，Nle-18，Tyr-34]bPTH-（1 -34）酰胺预孵育颅盖后，仍然存在一些反应性，尽管其水平显著低于在标准培养基中预孵育颅盖所观察到的水平。用bPTH-（3-34）（8 × 10−8 M）、[Nle-8，Nle-18，Tyr-34]bPTH-（3 - 34）酰胺（8 × 10 −8 M）或...预孵育颅盖骨后，未发现对bPTH-（l-84）的反应性有影响。
The time course of the stimulation of cAMP levels in embryonic rat calvaria by bovine parathyroid hormone- (1–84) [bPTH-(l–84)], bPTH-(l–34), and [Nle-8, Nle-18, Tyr-34]bPTH-(l–34) amide was studied at concentrations of 8 × 10−8 M. After addition of the hormone preparations, cAMP levels reached a maximum at 10 min and declined nearly to basal levels by 4 h. Incubation of rat calvaria for 2 h on a solid coagulum with the same concentration (8 × 10−8 M) of bPTH- (1–84) resulted in an almost complete loss of responsiveness (no significant further rise of the cAMP level) upon renewed addition of the hormone. After preincubation of the calvaria with bPTH- (1–34) or [Nle-8, Nle-18, Tyr-34]bPTH-(l–34) amide, some responsiveness remained, though at a level significantly less than that seen with calvaria preincubated in a standard medium. No effect on responsiveness to bPTH-(l–84) was found after preincubation of the calvaria with bPTH-(3–34) (8 × 10−8 M), [Nle-8, Nle-18, Tyr-34]bPTH-(3–34) amide (8 × 10−8 M), or ...