Molecular Basis of Transferrin Polymorphism in Goldfish (Carassius auratus)
Molecular Basis of Transferrin Polymorphism in Goldfish (Carassius auratus)
复制标题
DOI：
10.1023/b:gene.0000039855.55445.67
复制
发表时间：
2004-07
期刊：
影响因子：
1.5
通讯作者：
L. Yang;L. Zhou;J. Gui
中科院分区：
文献类型：
--
作者：
L. Yang;L. Zhou;J. Gui
研究了金鱼（Carassius auratus）颜色品种的转铁蛋白（TF）多态性，并分析了其分子基础。从金鱼近交系中鉴定出 3 个 TF 变体（A1、A2 和 B1），其中 A1 和 B1 在天然凝胶和 SDS-PAGE 凝胶上均表现出较大的电泳差异。从A1A1、A1B1和B1B1个体中克隆并测序了变体A1和B1对应的TFcDNA，并分析了其推导的氨基酸序列。变体 A1 和 B1 之间存在显着的氨基酸变异，肽长度、理论分子量 (Mw) 和等电点 (pI) 存在显着差异。在两个氨基酸序列中没有观察到潜在的糖基化位点，这排除了碳水化合物差异可能导致TF变体之间电泳变异的可能性。进一步分析表明，两种变体A1和B1在SDS-PAGE上的不同电泳迁移率是由于它们的Mw差异造成的，而天然PAGE的差异可以通过它们的pI变化来解释。此外，在 A1A1、A1B1 和 B1B1 个体中扩增含有转铁蛋白等位基因的基因组 DNA 片段并进行 RFLP 分析。数据揭示了每种 TF 基因型的特征带模式，并证明 TFalleles A1 和 B1 可以用作共显性标记系统。与金鱼 TF 变体相关的初步工作将有助于理解鱼类 TF 多态性的进化和功能意义。
Transferrin (TF) polymorphism was investigated in a color variety of goldfish (Carassius auratus), and its molecular basis analyzed. Three TF variants (A1, A2and B1) were identified from an inbred strain of the goldfish, of which A1and B1displayed a large electrophoretic difference on both native and SDS-PAGE gels. TheTFcDNAs corresponding to variants A1and B1were cloned and sequenced from A1A1, A1B1and B1B1individuals, and their deduced amino acid sequences were analyzed. Substantial amino acid variation occurred between variants A1and B1, with significant differences in peptide length, theoretical molecular weight (Mw) and isoelectric point (pI). No potential glycosylation sites were observed in the two amino acid sequences, which excluded the possibility that carbohydrate difference might cause electrophoretic variation among the TF variants. Further analysis suggested that the distinct electrophoretic mobility of the two variants A1and B1by SDS-PAGE resulted from their Mw difference, while the difference by the native PAGE could be explained by their pI variation. Furthermore, genomic DNA fragments containing the transferrin alleles were amplified and subjected to RFLP analysis in A1A1, A1B1and B1B1individuals. The data revealed characteristic banding patterns for each TF genotype, and demonstrated that theTFallelesA1andB1could be used as a co-dominant marker system. The initial work relating to the goldfish TF variants will benefit the understanding of the evolutionary and functional significance of TF polymorphism in fish.