In-depth sphingomyelin characterization using electron impact excitation of ions from organics and mass spectrometry
In-depth sphingomyelin characterization using electron impact excitation of ions from organics and mass spectrometry
复制标题
DOI：
10.1194/jlr.m067199
复制
发表时间：
2016-05-01
影响因子：
6.5
通讯作者：
Baker, Paul R. S.
中科院分区：
文献类型：
--
作者：
Baba, Takashi;Campbell, J. Larry;Baker, Paul R. S.
电子碰撞激发离子从有机物（EIEIO），也被称为电子诱导解离，施加到单电荷SM分子物种在气相中。使用ESI和配备有电子-离子反应装置的四极TOF质谱仪，我们发现SMs片段足以识别其脂质类、酰基结构和双键的位置。利用该技术，在牛乳、猪脑、鸡蛋黄和牛心四种天然脂质提取物中发现了近200种SM分子。除了最常见的骨架，d18：1，鞘氨醇与一系列的碳链长度，sphingadienes，和一些鞘氨醇骨架也被检测到。还鉴定了天然SM中的修饰，包括在碳-碳双键上添加碘/甲醇。这种无与伦比的新方法使用EIEIO-MS进行SM分析，显示出作为结构表征的独特而强大的工具的前景。
Electron impact excitation of ions from organics (EIEIO), also referred to as electron-induced dissociation, was applied to singly charged SM molecular species in the gas phase. Using ESI and a quadrupole TOF mass spectrometer equipped with an electron-ion reaction device, we found that SMs fragmented sufficiently to identify their lipid class, acyl group structure, and the location of double bond(s). Using this technique, nearly 200 SM molecular species were found in four natural lipid extracts: bovine milk, porcine brain, chicken egg yolk, and bovine heart. In addition to the most common backbone, d18:1, sphingosines with a range of carbon chain lengths, sphingadienes, and some sphinganine backbones were also detected. Modifications in natural SMs were also identified, including addition of iodine/methanol across a carbon-carbon double bond. This unparalleled new approach to SM analysis using EIEIO-MS shows promise as a unique and powerful tool for structural characterization.