Thymidine uptake and cell migration in cheek epithelium of zinc-deficient rats.
Thymidine uptake and cell migration in cheek epithelium of zinc-deficient rats.
复制标题
缺锌大鼠脸颊上皮的胸苷摄取和细胞迁移。
DOI：
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发表时间：
1973
期刊：
影响因子：
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通讯作者：
J. Meyer
中科院分区：
文献类型：
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作者：
O. Alvares;J. Meyer
.雄性断乳大鼠饲喂含1.3 mg/kg锌的饲料，对照组饲喂含40 mg /kg锌的饲料。通过腹膜内注射1 μCi氚化胸苷/g体重后1小时和3天处死的动物的标记细胞计数，获得颊上皮细胞分裂和向上迁移的速率。胸苷给药后1小时观察到对照组标记细胞数增加3.4倍，3天后增加2.7倍。对照组在第3天和第1小时的标记细胞比率为4.96，实验动物为3.99。在两组中，标记细胞在上皮细胞连续细分中的百分比分布是相同的。在实验动物中，颗粒层和角蛋白层交界处附近的标记细胞数量是对照组的2.9倍。
这表明，缺锌的颊上皮的大鼠可能是一个有用的模型与细胞增殖率增加的病理条件的研究。
. Male weanling rats were fed a diet containing 1.3 mg zinc/kg diet and controls the same diet supplemented by 40 mg /zinc/kg diet. The rates of cell division and upward migration in the cheek epithelium were obtained from counts of labelled cells in animals sacrificed one hour and three days following intraperitoneal injection of 1 μCi tritiated thymidine/g body weight. A 3.4-fold increase over the number of labelled cells in controls was observed one hour after thymidine administration and a 2.7-fold increase three days later. Ratios of labelled cells at three days and one hour were 4.96 in controls and 3.99 in experimental animals. The percentage distribution of labelled cells in successive subdivisions of epithelium was identical in both groups. The number of labelled cells near the junction of granular and keratin layers was 2.9-fold greater in the experimental animals.
It is suggested that the zinc-deficient cheek epithelium of the rat might be a useful model for the study of pathological conditions associated with increased rates of cell proliferation.