Evidence for DNA modification in the maintenance of X-chromosome inactivation of adult mouse tissues.
Evidence for DNA modification in the maintenance of X-chromosome inactivation of adult mouse tissues.
复制标题
DNA 修饰维持成年小鼠组织 X 染色体失活的证据。
DOI：
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发表时间：
1982
影响因子：
11.1
通讯作者：
R. Liskay
中科院分区：
文献类型：
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作者：
V. Chapman;P. Kratzer;L. Siracusa;B. A. Quarantillo;R. Evans;R. Liskay
利用哺乳动物细胞DNA转化技术，研究了DNA修饰在维持哺乳动物X染色体失活中的作用。无活性的X-染色体DNA从成年小鼠组织中的X-连锁的次黄嘌呤磷酸核糖转移酶基因（Hprt）的转化中发挥作用的能力，可以确定通过利用最近发现的电泳变异形式的次黄嘌呤磷酸核糖转移酶和以前可用的X：常染色体易位。我们的研究结果表明，从成年雌性小鼠的几个组织中的非活性X染色体DNA是非常低效的，在活性X染色体DNA相比，在引发次黄嘌呤磷酸核糖基转移酶的遗传转化。这些结果提供了在体内的证据，是一致的DNA修饰在维持X染色体失活的重要作用。
The role of DNA modification in the maintenance of mammalian X-chromosome inactivation was investigated by using the technique of DNA transformation in mammalian cells. The ability of inactive X-chromosome DNA from adult mouse tissues to act in transformation for the X-linked hypoxanthine phosphoribosyltransferase gene (Hprt) could be ascertained by utilizing a recently discovered electrophoretic variant form of the hypoxanthine phosphoribosyltransferase enzyme and a previously available X:autosome translocation. Our findings indicate that inactive X-chromosome DNA from several tissues of adult female mice is strikingly inefficient, in comparison to active X-chromosome DNA, in eliciting genetic transformation for hypoxanthine phosphoribosyltransferase. These results provide in vivo evidence that is consistent with DNA modification playing an important role in the maintenance of X-chromosome inactivation.