Evidence that dorsal-ventral differences in gap junctional communication in the early Xenopus embryo are generated by beta-catenin independent of cell adhesion effects.
Evidence that dorsal-ventral differences in gap junctional communication in the early Xenopus embryo are generated by beta-catenin independent of cell adhesion effects.
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有证据表明，早期非洲爪蟾胚胎间隙连接通讯的背腹差异是由 β-连环蛋白产生的，与细胞粘附效应无关。
DOI：
10.1006/dbio.1998.8951
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发表时间：
1998
影响因子：
2.7
通讯作者：
Heasman,J
中科院分区：
文献类型：
--
作者：
Krufka,A;Johnson,RG;Wylie,CC;Heasman,J
Gap junctional communication (GJC) is regulated in the earlyXenopusembryo and quantitative differences in junctional communication correlate with the specification of the dorsal–ventral axis. To address the mechanism that is responsible for regulating this differential communication, we investigated the function of β-catenin during the formation of the dorsal–ventral axis inXenopusembryos by blocking its synthesis with antisense oligodeoxynucleotides. This method has previously been shown to reduce the level of β-catenin in the early embryo, prior to zygotic transcription, and to inhibit the formation of the dorsal axis (Heasmanet al., 1994,Cell79, 791–803). We show here that antisense inhibition of β-catenin synthesis also reduces GJC among cells in the dorsal hemisphere of 32-cell embryos to levels similar to those observed among ventral cells. Full-length β-catenin mRNA can restore elevated levels of dorsal GJC when injected into β-catenin-deficient oocytes, demonstrating the specificity of the β-catenin depletion with the antisense oligonucleotides. Thus, endogenous β-catenin is required for the observed differential GJC. This regulation of GJC is the earliest known action of the dorsal regulator, β-catenin, inXenopusdevelopment. Two lines of evidence, presented here, indicate that β-catenin acts within the cytoplasm to regulate GJC, rather than through an effect on cell adhesion. First, when EP-cadherin is overexpressed and increased adhesion is observed, embryos display both a ventralized phenotype and reduced dye transfer. Second, a truncated form of β-catenin (i.e., the ARM region), that lacks the cadherin-binding domain, restores dorsal GJC to β-catenin-depleted embryos. Thus, β-catenin appears to regulate GJC independent of its role in cell–cell adhesion, by acting within the cytoplasm through a signaling mechanism.
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DOI：
10.1083/jcb.134.5.1283
发表时间：
1996-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Orsulic S;Peifer M
通讯作者：
Peifer M
母体α-连环蛋白和斑珠蛋白在早期非洲爪蟾胚胎中的作用。
DOI：
10.1242/dev.124.8.1553
发表时间：
1997
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Kofron,M;Spagnuolo,A;Klymkowsky,M;Wylie,C;Heasman,J
通讯作者：
Heasman,J
补充胆固醇后培养细胞之间的间隙连接组装增加。
影响因子：
4
作者：
Meyer,R;Malewicz,B;Baumann,WJ;Johnson,RG
通讯作者：
Johnson,RG
Wnt-1、激活素 B 和 bFGF 异位表达对 32 细胞非洲爪蟾胚胎间隙连接通透性的明显影响。
影响因子：
2.7
作者：
Olson,DJ;Moon,RT
通讯作者：
Moon,RT
DOI：
10.1073/pnas.85.19.7274
发表时间：
1988-10-01
影响因子：
11.1
作者：
MEGE, RM;MATSUZAKI, F;EDELMAN, GM
通讯作者：
EDELMAN, GM