Metabolism of pectin in rumen bacteria Butyrivibrio fibrisolvens and Prevotella ruminicola
Metabolism of pectin in rumen bacteria Butyrivibrio fibrisolvens and Prevotella ruminicola
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DOI：
10.1046/j.1472-765x.1999.00671.x
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发表时间：
1999-12-01
影响因子：
2.4
通讯作者：
Dusková, D
中科院分区：
文献类型：
--
作者：
Marounek, M;Dusková, D
与在L-阿拉伯糖和D-葡萄糖上生长的相应培养物相比，在果胶上生长的溶纤维丁酸弧菌787和瘤胃普雷沃氏菌AR 29的培养物产生显著更多的乙酸盐和更少的丁酸盐、乳酸盐、琥珀酸盐和氢。在这两种细菌中，果胶和阿拉伯糖的发酵产生的乳酸比葡萄糖的发酵少。这些菌株的果胶生长的细胞具有2-酮-3-脱氧-6-磷酸葡萄糖酸醛缩酶（EC 4.1.2.14）的活性，该酶催化Entner-Doudoroff途径中的丙酮酸形成。果糖二磷酸醛缩酶（EC 4.1.2.13）的活性在葡萄糖和果胶上培养的细胞中均发现。在所研究的瘤胃细菌菌株中未检测到磷酸酮醇酶活性（EC 4.1.2.9）。
Cultures Of Butyrivibrio fibrisolvens 787 and Prevotella ruminicola AR29 grown on pectin produced significantly more acetate and less butyrate, lactate, succinate and hydrogen than corresponding cultures grown on L-arabinose and D-glucose. In both bacteria, fermentation of pectin and arabinose yielded less lactate than fermentation of glucose. Pectin-grown cells of these strains possessed activity of 2-keto-3-deoxy-6-phosphogluconate aldolase (EC 4.1.2.14), an enzyme catalysing the pyruvate formation in the Entner-Doudoroff pathway. The activity of fructosediphosphate aldolase (EC 4.1.2.13) mas found both in cells cultivated on glucose and on pectin. The phosphoketolase activity (EC 4.1.2.9) could not be detected in rumen bacterial strains studied.