Development of a Sensitive Immunochromatographic Method Using Lanthanide Fluorescent Microsphere for Rapid Serodiagnosis of COVID-19
Development of a Sensitive Immunochromatographic Method Using Lanthanide Fluorescent Microsphere for Rapid Serodiagnosis of COVID-19
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DOI：
10.1021/acssensors.0c00927
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发表时间：
2020-08-28
期刊：
影响因子：
8.9
通讯作者：
Cheng, Xunjia
中科院分区：
文献类型：
--
作者：
Feng, Meng;Chen, Jun;Cheng, Xunjia
导致COVID-19大流行的SARS-CoV-2感染在两个月内迅速在全球传播。快速诊断和隔离患者是预防和控制COVID-19传播的有效途径。为此，建立了一种灵敏的免疫荧光检测方法，可在10 min内快速检测人血清中COVID-19特异性IgM和IgG。以2019新型冠状病毒重组核衣壳蛋白为捕获抗原。采用镧系铕（III）荧光微球进行固相免疫层析定量测定。共纳入28例临床阳性和77例阴性血清或血浆样本。通过对COVID-19患者和健康人血清或血浆的分析，计算出免疫层析法的灵敏度和特异性分别为98.72%和100% （IgG）和98.68%和93.10% （IgM）。结果表明，快速免疫分析法具有较高的敏感性和特异性，可用于新型冠状病毒肺炎的快速血清诊断。
The SARS-CoV-2 infection that caused the COVID-19 pandemic quickly spread worldwide within two months. Rapid diagnosis of the disease and isolation of patients are effective ways to prevent and control the spread of COVID-19. Therefore, a sensitive immunofluorescent assay method was developed for rapid detection of special IgM and IgG of COVID-19 in human serum within 10 min. The recombinant nucleocapsid protein of 2019 novel coronavirus was used as capture antigen. Lanthanide, Eu(III) fluorescent microsphere, was used to qualitatively/semiquantitatively determine the solid phase immunochromatographic assay. A total of 28 clinical positive and 77 negative serum or plasma samples were included in the test. Based on the analysis of serum or plasma from COVID-19 patients and healthy people, the sensitivity and specificity of the immunochromatographic assay were calculated as 98.72% and 100% (IgG), and 98.68% and 93.10% (IgM), respectively. The results demonstrated that rapid immunoassay has high sensitivity and specificity and was useful for rapid serodiagnosis of COVID-19.