PROTEIN KINASE-C-ZETA ISOFORM IS CRITICAL FOR MITOGENIC SIGNAL-TRANSDUCTION
PROTEIN KINASE-C-ZETA ISOFORM IS CRITICAL FOR MITOGENIC SIGNAL-TRANSDUCTION
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DOI：
10.1016/0092-8674(93)80056-k
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发表时间：
1993-08-13
期刊：
影响因子：
64.5
通讯作者：
MOSCAT, J
中科院分区：
文献类型：
--
作者：
BERRA, E;DIAZMECO, MT;MOSCAT, J
蛋白激酶C zeta（zetaPKC）对卵细胞成熟的要求是胰岛素、p21ras和磷脂酰胆碱水解磷脂酶C的反应。在这里，我们提供的实验证据表明，zetaPKC的激活不仅是必要的，而且本身也是充分的，以激活卵母细胞的成熟，并产生对小鼠成纤维细胞生长控制的解除。此外，通过使用显性激酶缺陷突变体zetaPKC，我们证实该激酶是卵母细胞和成纤维细胞有丝分裂激活所必需的。这些结果允许我们提出zetaPKC作为p21ras下游有丝分裂信号转导的关键步骤。
The requirement of protein kinase C zeta(zetaPKC) for maturation of X. laevis oocytes in response to insulin, p21ras, and phosphatidylcholine-hydrolyzing phospholipase C has recently been shown. Here we present experimental evidence demonstrating that activation of zetaPKC is not only necessary but also sufficient by itself to activate maturation in oocytes and to produce deregulation of growth control in mouse fibroblasts. Furthermore, by using a dominant kinase-defective mutant zetaPKC, we confirm that this kinase is required for mitogenic activation in oocytes and fibroblasts. These results permit us to propose zetaPKC as a critical step downstream of p21ras in mitogenic signal transduction.