The use of pHluorins for optical measurements of presynaptic activity
The use of pHluorins for optical measurements of presynaptic activity
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DOI：
10.1016/s0006-3495(00)76468-x
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发表时间：
2000-10-01
影响因子：
3.4
通讯作者：
Ryan, TA
中科院分区：
文献类型：
--
作者：
Sankaranarayanan, S;De Angelis, D;Ryan, TA
用于光学测量突触前活动的遗传编码的报告者对于测量完整或半完整神经元网络内的神经传递具有重要的前景。我们的特点是pH敏感的绿色荧光蛋白为基础的传感器（pHluorins）的突触囊泡循环在神经末梢。荧光蛋白具有类似于7.1的pK，这使得它们对于在动作电位期间循环通过质膜时跟踪突触囊泡腔pH是理想的。使用这种方法的预期信号的理论分析和指导方针，为今后的记者发展。
Genetically encoded reporters for optical measurements of presynaptic activity hold significant promise for measurements of neurotransmission within intact or semi-intact neuronal networks. We have characterized pH-sensitive green fluorescent protein-based sensors (pHluorins) of synaptic vesicle cycling at nerve terminals. pHluorins have a pK similar to 7.1, which make them ideal for tracking synaptic vesicle lumen pH upon cycling through the plasma membrane during action potentials. A theoretical analysis of the expected signals using this approach and guidelines for future reporter development are provided.