Diversity-Oriented Enzymatic Modular Assembly of ABO Histo-blood Group Antigens
Diversity-Oriented Enzymatic Modular Assembly of ABO Histo-blood Group Antigens
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ABO 组织血型抗原的多样性酶促模块化组装
DOI：
10.1021/acscatal.6b02755
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发表时间：
2016
期刊：
影响因子：
12.9
通讯作者：
曹鸿志
中科院分区：
文献类型：
--
作者：
叶金凤;刘现伟;彭鹏;易文;Xi Chen;王凤山;曹鸿志
使用面向多样性的酶模块组装(EMA)策略，实现了所有15种自然产生的人类ABH抗原的酶合成。开发了三个酶模块，每个一锅多酶模块包括一个糖基转移酶和一个或两个相应的糖核苷酸产生酶(S)。这些多酶串联过程提供了一个有效和方便的平台，通过三个操作简单的步骤从五个现成的双糖受体和三个简单的游离糖作为供体前体，集体合成所有15个ABH抗原。
Enzymatic synthesis of all 15 naturally occurring human ABH antigens was achieved using a diversity-oriented enzymatic modular assembly (EMA) strategy. Three enzyme modules were developed, each one-pot multienzyme module comprises a glycosyltransferase and one or two corresponding sugar nucleotide generating enzyme(s). These multienzyme cascade processes provide an efficient and convenient platform for collective synthesis of all 15 ABH antigens in three operationally simple steps from five readily available disaccharide acceptors and three simple free sugars as donor precursors.