HPLC-MS with Glass Chips Featuring Monolithically Integrated Electrospray Emitters of Different Geometries
HPLC-MS with Glass Chips Featuring Monolithically Integrated Electrospray Emitters of Different Geometries
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DOI：
10.1021/acs.analchem.5b04583
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发表时间：
2016-03-01
影响因子：
7.4
通讯作者：
Belder, Detlev
中科院分区：
文献类型：
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作者：
Lotter, Carsten;Heiland, Josef J.;Belder, Detlev
我们提出并评估了一种通过完全集成的电喷雾发射器将玻璃芯片中的液相色谱与质谱联用的方法。我们开发了一个仪器平台，它允许一个强大的和可重复的操作的高效芯片色谱耦合到质谱。不同形状的发射器的比较，从平的边缘上拉的几何形状，显示所有类型的典型的纳米HPLC流速同样表现良好。在低于50 nL.min（-1）的非常低的流速下，非常尖锐的、拉动的纳米喷雾发射器对于产生稳定的电喷雾是强制性的。
We present and evaluate an approach for coupling liquid chromatography in glass chips with mass spectrometry via fully integrated electrospray emitters. We developed an instrumental platform which allows a robust and reproducible operation of high performance chip chromatography coupled to mass spectrometry. A comparison of differently shaped emitters, from flat over edged to pulled geometries, revealed that all types performed equally well for typical nano-HPLC flow rates. At very low flow rates below 50 nL.min(-1) very sharp, pulled nanospray emitters turned out to be mandatory for the generation of a stable electrospray.