Polarized Raman Spectroscopy for Determining the Orientation of di-D-phenylalanine Molecules in a Nanotube.
Polarized Raman Spectroscopy for Determining the Orientation of di-D-phenylalanine Molecules in a Nanotube.
复制标题
偏振拉曼光谱法，用于确定纳米管中二苯甲酰氨酸分子的方向。
DOI：
10.1002/jrs.4884
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发表时间：
2016-09
影响因子：
2.5
通讯作者：
Lednev, Igor K.
中科院分区：
文献类型：
--
作者：
Sereda, Valentin;Ralbovsky, Nicole M.;Vasudev, Milana C.;Naik, Rajesh R.;Lednev, Igor K.
关键词：
短肽自组装成纳米结构已成为自下而上制备纳米材料的重要策略。对此类基于肽的构建块的显着兴趣是由于有机会控制结构良好的纳米管、纳米原纤维和水凝胶的结构和性质。结构生物学的两个主要工具X射线晶体学和溶液NMR在应用于肽纳米管时具有显著的局限性，因为它们的非晶体结构和大的重量。利用偏振拉曼光谱对D-联苯丙氨酸纳米管进行了结构表征。确定所选化学基团相对于纳米管主轴的取向。具体来说，CNH 3+基团的C-N键平行于纳米管轴，肽的羰基与轴倾斜约54°，COO−基团垂直于轴。确定的化学基团的取向允许理解的D-二苯基丙氨酸分子的取向是一致的，其平衡构象。所得数据表明，相对于纳米管主轴，D-二苯基丙氨酸分子只有一个取向。
Self-assembly of short peptides into nanostructures has become an important strategy for the bottom-up fabrication of nanomaterials. Significant interest to such peptide-based building blocks is due to the opportunity to control the structure and properties of well-structured nanotubes, nanofibrils, and hydrogels. X-ray crystallography and solution NMR, two major tools of structural biology, have significant limitations when applied to peptide nanotubes because of their non-crystalline structure and large weight. Polarized Raman spectroscopy was utilized for structural characterization of well-aligned D-Diphenylalanine nanotubes. The orientation of selected chemical groups relative to the main axis of the nanotube was determined. Specifically, the C-N bond of CNH3+groups is oriented parallel to the nanotube axis, the peptides’ carbonyl groups are tilted at approximately 54° from the axis and the COO− groups run perpendicular to the axis. The determined orientation of chemical groups allowed the understanding of the orientation of D-diphenylalanine molecule that is consistent with its equilibrium conformation. The obtained data indicate that there is only one orientation of D-diphenylalanine molecules with respect to the nanotube main axis.
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影响因子：
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