Non-photochemical quenching of chlorophyll fluorescence in Chlorella fusca acclimated to constant and dynamic light conditions
Non-photochemical quenching of chlorophyll fluorescence in Chlorella fusca acclimated to constant and dynamic light conditions
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DOI：
10.1023/a:1021230601077
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发表时间：
2002-01-01
影响因子：
3.7
通讯作者：
Mur, LR
中科院分区：
文献类型：
--
作者：
Garcia-Mendoza, E;Matthijs, HCP;Mur, LR
叶绿素荧光的非光化学猝灭（NPQ）涉及光能在光合机构中通过许多生理上不同的过程耗散。研究了6种不同光适应条件下的绿色小球藻（Chlorella fusca）的NPQ、叶黄素循环色素的脱环氧化态和状态转换之间的关系。10 h光照和14 h黑暗，所有培养物均服从周期性。三个文化收到了高的每日总辐照度，其他三个低。高和低辐照度分别在三种不同的模式下，在恒定的供应，正弦形状的强度调制，或作为一个正弦叠加振荡。在恒定的供应模式，但不是正弦和振荡模式，高光呈现三倍高的叶黄素循环色素含量比低光。叶黄素循环色素的动态相互转化仅限于强光培养。NPQ遵循光供应模式的动力学，并且在高光培养物中最高。在弱光培养中，NPQ主要与状态转换相关。这些观察结果得到了用二硫苏糖醇处理的样品进行的实验的支持。本文以高等植物为例，讨论了不同光照条件下绿色藻类中叶黄素循环和状态转换对NPQ的相对影响。
Non-photochemical quenching of chlorophyll fluorescence (NPQ) involves dissipation of light energy in the photosynthetic apparatus via a number of physiologically distinct processes. The relationships among NPQ, the (de)epoxidation state of the xanthophyll cycle pigments and state transitions was studied in the green alga Chlorella fusca, acquired from six differently light-acclimated continuous cultures. A 10 h light and 14 h darkness, periodicity was obeyed in all cultures. Three cultures received a high total daily irradiance, three others a low one. High and low irradiances were each dosed in three different modes at constant supply, with sine shape intensity modulation, or as a sine with superimposed oscillations. In the constant supply mode, but not for the sine and oscillating modes, high-light rendered a three-fold higher xantophyll cycle pigment content than low-light. Dynamic interconversion of xantophyll cycle pigments was restricted to high-light cultures. NPQ followed the kinetics of the light supply mode and was highest in high light cultures. In low-light cultures, NPQ correlated mainly to state transitions. These observations were supported by experiments with dithiothreithol-treated samples. The relative impact of xantophyll cycle operation and state transitions on NPQ in green algae from different light climates will be discussed with reference to higher plants.