Gene expression of keratinocyte and hepatocyte growth factors during the healing of rat gastric mucosal lesions.
Gene expression of keratinocyte and hepatocyte growth factors during the healing of rat gastric mucosal lesions.
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大鼠胃粘膜病变愈合过程中角质形成细胞和肝细胞生长因子基因的表达
DOI：
10.1016/0016-5085(95)90564-2
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发表时间：
1995
期刊：
影响因子：
29.4
通讯作者：
A. Nakamura
中科院分区：
文献类型：
--
作者：
Y. Kinoshita;H. Nakata;S. Hassan;M. Asahara;C. Kawanami;Y. Matsushima;Y. Naribayashi;C. Ping;D. Min;A. Nakamura
背景与目的胃粘膜愈合过程中刺激胃粘膜细胞生长的因素尚不完全清楚。本研究旨在探讨角质形成细胞生长因子(KGF)和肝细胞生长因子(HGF)在胃粘膜损伤愈合过程中的基因表达。方法采用Northern印迹分析和逆转录-聚合酶链式反应(RT-PCR)检测HGF和KGF信使RNA的表达。消炎痛诱导急性胃粘膜损伤时，HGF信使RNA在粘膜下层、肌层和浆膜层的表达增强，而KGF的转录本不受影响。大鼠胃粘膜上皮细胞株RGM1和原代培养的大鼠胃粘膜细胞与HGF或KGF共同孵育后，其增殖能力增强。结论在胃粘膜愈合过程中，HGF基因表达增加，KGF持续产生。
Background & AimsThe factors that stimulate the growth of gastric mucosal cells during gastric mucosal healing are not completely understood. This study was designed to investigate the gene expression of keratinocyte growth factor (KGF) and hepatocyte growth factor (HGF) in the healing of gastric mucosal lesions.MethodsNorthern blot analysis and reverse-transcription polymerase chain reaction were used to show HGF and KGF messenger RNA.ResultsTranscripts of HGF and KGF were not shown in rat gastric mucosal epithelium but were found in submucosal and muscular layers under normal conditions. When acute gastric mucosal lesions were induced by indomethacin treatment, expression of HGF messenger RNA was augmented in submucosal, muscular, or serosal layers, whereas the transcript of KGF was not influenced. When rat gastric mucosal epithelial cell line RGM1 and rat gastric mucosal primary culture cells were incubated with HGF or KGF, their proliferation was enhanced.ConclusionsThe results showed increased gene expression of HGF together with constant production of KGF during gastric mucosal healing.
影响因子：
15.9
作者：
PANOS, RJ;RUBIN, JS;MASON, RJ
通讯作者：
MASON, RJ
DOI：
10.1172/jci115189
发表时间：
1991-05
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Monica C. Chen;Alexander T. Lee;A. H. Soll
通讯作者：
Monica C. Chen;Alexander T. Lee;A. H. Soll