A pectin glucuronyltransferase gene is essential for intercellular attachment in the plant meristem
A pectin glucuronyltransferase gene is essential for intercellular attachment in the plant meristem
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DOI：
10.1073/pnas.252530499
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发表时间：
2002-12-10
影响因子：
11.1
通讯作者：
Satoh, S
中科院分区：
文献类型：
--
作者：
Iwai, H;Masaoka, N;Satoh, S
细胞间附着是多细胞生物形态发生过程中的一个关键步骤。通过利用单倍体紫花烟草叶盘培养进行T - DNA转化，产生了一种独特的突变体nolac - H18（具有松散附着细胞的非器官发生性愈伤组织），它丧失了形成紧密细胞间附着和不定芽的能力。被T - DNA标记的基因名为NpGUT1（葡萄糖醛酸基转移酶1），与动物葡萄糖醛酸基转移酶的催化结构域基因相似，并且主要在茎尖和根尖分生组织中表达。NpGUT1的转化弥补了nolac - H18突变，而反义NpGUT1 RNA的表达产生了皱缩的茎。该突变导致果胶中鼠李半乳糖醛酸聚糖II的葡萄糖醛酸出现缺陷，这极大地减少了鼠李半乳糖醛酸聚糖II的硼酸盐交联形成。NpGUT1编码一种独特的葡萄糖醛酸基转移酶，是在果胶生物合成中鉴定出的一种糖基转移酶基因，对植物分生组织和组织中的细胞间附着至关重要。
Intercellular attachment is an essential process in the morphogenesis of multicellular organisms. A unique mutant, nolac-H18 (nonorganogenic callus with loosely attached cells), generated by T-DNA transformation using leaf-disk cultures of haploid Nicotiana plumbaginifolia, lost the ability to form tight intercellular attachments and adventitious shoots. The gene tagged with T-DNA, named NpGUT1 (glucuronyltransferase 1), was similar to the gene for the catalytic domains of animal glucuronyltransferases and was expressed predominantly in shoot and root apical meristems. The transformation of NpGUT1 complemented the nolac-H18 mutation, and the expression of antisense NpGUT1 RNA produced crumbled shoots. The mutation caused defects in the glucuronic acid of rhamnogalacturonan II of pectin, which drastically reduced the formation of borate cross-linking of rhamnogalacturonan II. NpGUT1, which encodes a unique glucuronyltransferase, is a glycosyltransferasegene identified in pectin biosynthesis and is essential for intercellular attachment in plant meristems and tissues.