Genotoxicity Evaluation of Nanomaterials: DNA Damage, Micronuclei, and 8-Hydroxy-2-deoxyguanosine Induced by Magnetic Doped CdSe Quantum Dots in Male Mice
Genotoxicity Evaluation of Nanomaterials: DNA Damage, Micronuclei, and 8-Hydroxy-2-deoxyguanosine Induced by Magnetic Doped CdSe Quantum Dots in Male Mice
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DOI：
10.1021/tx2000015
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发表时间：
2011-05-01
影响因子：
4.1
通讯作者：
Rao, K. V.
中科院分区：
文献类型：
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作者：
Khalil, W. K. B.;Girgis, E.;Rao, K. V.
量子点（QD）是一类新型的无机荧光团，由于其优异的物理化学性质及其作为生物标志物和分子生物医学成像的应用而获得广泛的认可。本研究的目的是评估暴露于平均尺寸为5.0 +/- 0.2 nm的CdSe量子点和掺杂有1%钴离子的类似尺寸的CdSe的小鼠体内遗传毒性。使用巯基乙酸（MAA）对量子点进行表面改性，以使其具有生物相容性和水溶性。将MAA-QD以按MAA-QD重量计500、1000和2000 mg/kg的剂量口服给予小鼠。在处理2天和7天后收集骨髓和肝脏样品。结果表明，高剂量的MAA-QDs处理2 d后，能明显诱导DNA损伤、微核（MN）形成和DNA加合物（8-羟基-2-脱氧鸟苷，8-OHdG）的生成。然而，在处理7天后，未掺杂的MAA-QD（2000 mg/kg）和掺杂的MAA-QD（1000和2000 mg/kg）都观察到DNA损伤和MN形成的频率以及DNA加合物的产生增加。我们的研究结果表明，暴露于高剂量的纯MAA-QD或掺杂钴的MAA-QD有可能导致间接的体内遗传损伤，这可能归因于小鼠中自由基诱导的氧化应激。
Quantum dots (QDs) are a novel class of inorganic fluorophores which are gaining widespread recognition as a result of their exceptional photophysical properties and their applications as a biomarker and in molecular biomedical imaging. The aim of this study was to evaluate the in vivo genotoxicity in mice exposed to CdSe quantum dots of average size 5.0 +/- 0.2 nm and CdSe doped with 1% cobalt ions of similar size. The quantum dots are surface modified using mercaptoacetic acid (MAA) in order to be biocompatible and water-soluble. The MAA-QDs were given to the mice orally at doses of 500, 1000, and 2000 mg/kg by weight of MAA-QDs. Bone marrow and liver samples were collected after two and seven days of treatment. The results indicated that after two days of treatment, the high dose of doped MAA-QDs was significantly able to induce DNA damage, formation of micronuclei (MNs), and generation of DNA adduct (8-hydroxy-2-deoxyguanosine, 8-OHdG). However, increasing DNA damage and the frequency of MNs formation as well as the generation of DNA adducts were observed with both the undoped MAA-QDs (2000 mg/kg) and doped MAA-QDs (1000 and 2000 mg/kg) after seven days of treatment. The results of our study indicate that exposure to high doses of pure MAA-QDs or MAA-QDs doped with cobalt has the potential to cause indirect in vivo genetic damage, which may be attributed to free radical-induced oxidative stress in mice.