Alterations in microRNA Expression in a Murine Model of Diet-Induced Vasculogenic Erectile Dysfunction
Alterations in microRNA Expression in a Murine Model of Diet-Induced Vasculogenic Erectile Dysfunction
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DOI：
10.1111/jsm.12793
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发表时间：
2015-03-01
影响因子：
3.5
通讯作者：
Lysiak, Jeffrey J.
中科院分区：
文献类型：
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作者：
Barbery, Carlos E.;Celigoj, Frank A.;Lysiak, Jeffrey J.
微RNA（miRs）是非编码的，本研究的目的是鉴定在躯体组织（CT）中表达的miRs，并确定miRs在饮食诱导的勃起功能障碍（艾德）小鼠模型中是否表现出差异表达。采用可定量的miR谱分析技术（NanoString）测定超过600个miR的表达。主要结果测量使用负二项回归模型对基于计数的数据进行差异表达分析。测定平均表达水平、倍数变化和假发现校正的P值。结果在对照小鼠中，NanoString分析显示181个miRs表达高于背景水平，5个miRs高水平表达。与对照饮食的小鼠相比，饮食诱导的艾德导致CT中6个miR的上调和65个miR的下调。关注上调的miR，我们选择了五个用于Q-PCR验证。结论miR-720、miR-1937 a、miR-205可能在CT的mRNA调控中发挥重要作用，miR-151- 5 p和miR-1937 c可能参与饮食诱导的血管性ED。Barbery CE、Celigoj FA、Turner SD、Smith RP、Kavoussi PK、Annex BH和Lysiak JJ。饮食诱导的血管性勃起功能障碍小鼠模型中microRNA表达的改变J Sex Med 2015;12：621-630.
IntroductionMicroRNAs (miRs) are noncoding, endogenous RNA molecules that regulate gene expression and play roles in response to vascular injury.AimThe aim of this study was to identify miRs expressed in corporal tissue (CT) and to determine whether miRs demonstrate differential expression in a mouse model of diet-induced erectile dysfunction (ED).MethodsRNA was isolated from the CT from control mice and mice with diet-induced ED. A quantifiable miR profiling technique (NanoString) was used to determine the expression of over 600miRs.Main Outcome MeasuresDifferential expression analysis was performed using a negative binomial regression model for count-based data. Mean expression levels, fold change, and false discovery-corrected P values were determined. Candidate miRs were validated via quantitative polymerase chain reaction (Q-PCR).ResultsIn control mice, NanoString analysis revealed that 181miRs were expressed above background levels and 5miRs were expressed at high levels. Diet-induced ED resulted in the up-regulation of 6miRs and the down-regulation of 65miRs in the CT compared with mice on control diet. Focusing on the upregulated miRs, we chose five for Q-PCR validation. Of these five, two (miR-151-5p and miR-1937c) demonstrated significance via Q-PCR, whereas the other three (miR-720, miR-1937a, miR-205) trended in the correct direction.ConclusionsMiRs may play a significant role in mRNA regulation in CT and specific miRs may be involved in diet-induced vasculogenic ED. Future studies are aimed at determining the mRNA targets of these miRs. Barbery CE, Celigoj FA, Turner SD, Smith RP, Kavoussi PK, Annex BH, and Lysiak JJ. Alterations in microRNA expression in a murine model of diet-induced vasculogenic erectile dysfunction. J Sex Med 2015;12:621-630.