Manganese-enhanced MRI of the mouse auditory pathway
Manganese-enhanced MRI of the mouse auditory pathway
复制标题
DOI：
10.1002/mrm.21645
复制
发表时间：
2008-07-01
影响因子：
3.3
通讯作者：
Michaelis, Thomas
中科院分区：
文献类型：
--
作者：
Watanabe, Takashi;Frahm, Jens;Michaelis, Thomas
在小鼠体内首次使用锰增强MRI(2.35T，3DFlash，117µm各向同性分辨率)完成了作为听觉通路一部分的外侧丘系和上橄榄复合体的功能定位。当动物暴露在声音刺激下48小时后，全身注射氯化锰后，这些和脑干中的其他听觉中枢呈现出明显的信号增强，但在安静的环境中则没有。结果表明，锰在由耳蜗核、上橄榄复合体、外侧丘系和下丘组成的神经回路中存在激活依赖性的蓄积。外侧丘系的显著增强表明，与刺激相关的锰积累不仅反映了细胞外液的局部摄取，而且反映了听觉系统内轴突运输的同步输送。
Functional mapping of the lateral lemniscus and the superior olivary complex as part of the auditory pathway was accomplished for the first time in mice in vivo using manganese-enhanced MRI (2.35T, 3D FLASH, 117 mu m isotropic resolution). These and other auditory centers in the brainstem presented with pronounced signal enhancements after systemic administration of manganese chloride when animals were exposed to acoustic stimuli for 48 hr, but not when kept in a quiet environment. The results indicate an activation-dependent accumulation of manganese in the neural circuit composed of the cochlear nucleus, the superior olivary complex, the lateral lemniscus, and the inferior colliculus. The marked enhancement of the lateral lemniscus suggests that the stimulus-related accumulation of manganese reflects not only a regional uptake from extracellular fluid but also a concurrent delivery by axonal transport within the auditory system.