Expression of mumps virus glycoproteins in mammalian cells from cloned cDNAs: both F and HN proteins are required for cell fusion.
Expression of mumps virus glycoproteins in mammalian cells from cloned cDNAs: both F and HN proteins are required for cell fusion.
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通过克隆的 cDNA 在哺乳动物细胞中表达腮腺炎病毒糖蛋白：F 和 HN 蛋白都是细胞融合所必需的。
DOI：
10.1016/0042-6822(92)90482-5
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发表时间：
1992
期刊：
影响因子：
3.7
通讯作者：
A. Yamada
中科院分区：
文献类型：
--
作者：
K. Tanabayashi;K. Takeuchi;K. Okazaki;M. Hishiyama;A. Yamada
将腮腺炎病毒融合蛋白（F）或血凝素-神经氨酸酶（HN）蛋白基因的cDNA插入pcDL-SRα表达载体中，构建了两个重组质粒。F和HN蛋白在用各自的重组质粒转染的COS 7细胞中表达，与在腮腺炎病毒感染的细胞中合成的对应物在电泳迁移率方面是不可区分的。F蛋白被切割并在细胞表面表达，但也检测到未切割的形式。表达的FIN蛋白被转运到细胞表面并吸附豚鼠红细胞。当两种重组质粒DNA一起转染COS 7细胞时，可诱导合胞体形成，但仅携带F基因的重组质粒不能诱导合胞体形成。这一观察结果表明，腮腺炎病毒介导的细胞融合需要F和HN糖蛋白。
Two recombinant plasmids were constructed by inserting the cDNAs of either the fusion (F) or the hemagglutinin-neuraminidase (HN) protein genes of mumps virus into the pcDL-SRα expression vector. Both the F and the HN proteins expressed in COS7 cells transfected with their respective recombinant plasmids were indistinguishable in terms of electrophoretic mobility from their counterparts synthesized in mumps virus-infected cells. The F protein was cleaved and expressed on the cell surface, but uncleaved forms were also detected. The expressed FIN protein was transported to the cell surface and adsorbed guinea pig erythrocytes. Syncytium formation was induced when COS7 cells were transfected with both recombinant plasmid DNAs together, but not with the recombinant plasmid only carrying the F gene. This observation indicates that cell fusion mediated by mumps virus requires both the F and the HN glycoproteins.
鉴定参与腮腺炎病毒血凝素神经氨酸酶蛋白唾液酸酶活性的氨基酸。
影响因子：
3.7
作者：
Waxham,MN;Aronowski,J
通讯作者：
Aronowski,J