Effect of UV-B radiation and a supplement of CaCl2 on carotenoid biosynthesis in germinated corn kernels
Effect of UV-B radiation and a supplement of CaCl2 on carotenoid biosynthesis in germinated corn kernels
复制标题
UV-B 辐射和 CaCl2 补充对发芽玉米粒中类胡萝卜素生物合成的影响
DOI：
10.1016/j.foodchem.2018.11.089
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发表时间：
2019-04-25
期刊：
影响因子：
8.8
通讯作者：
Li, Dajing
中科院分区：
文献类型：
--
作者：
He, Weiwei;Wang, Yuxi;Li, Dajing
研究了UV-B辐射和CaCl 2对萌发玉米籽粒类胡萝卜素含量的影响。UV-B辐射和CaCl 2处理均能有效提高类胡萝卜素含量和抗氧化活性。UV-B辐射与CaCl 2复合处理的类胡萝卜素含量较高。UV-B辐射与CaCl 2复合处理比单独处理提高了超氧化物歧化酶和过氧化物酶的活性，降低了丙二醛的含量。在UV-B辐射和CaCl 2处理下，类胡萝卜素合成途径相关基因PSY、PDS、ZDS、LCYB、LCYE、BCH 1、CYP 97 C的mRNA表达呈现出不同的模式。这说明UV-B辐射能提高番茄类胡萝卜素含量和抗氧化酶活性。此外，CaCl 2还能进一步提高类胡萝卜素含量，降低UV-B辐射引起的光氧化损伤。
This study investigated effects of UV-B radiation and CaCl2 on the enhancement of carotenoid content in germinated corn kernels. UV-B radiation and CaCl2 treatments were effective for promoting both carotenoid content and antioxidant activity. Furthermore, the carotenoid content was greater when under the combined treatments of UV-B radiation and CaCl2. Activities of superoxide dismutase and peroxidase were enhanced but malondialdehyde content was weakened by UV-B radiation plus CaCl2 compared to the UV-B radiation only. The mRNA expression of PSY, PDS, ZDS, LCYB, LCYE, BCH1, CYP97C genes involved in the carotenoid biosynthesis pathway showed different patterns in UV-B radiation and CaCl2 treatments. This reveals that the UV-B radiation can increase carotenoid content and antioxidant enzyme activity. Moreover, CaCl2 can further improve carotenoid content and reduce photooxidative damage caused by UV-B radiation.