Micro-CT scouting for transmission electron microscopy of human tissue specimens.
Micro-CT scouting for transmission electron microscopy of human tissue specimens.
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DOI：
10.1111/jmi.12385
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发表时间：
2016-07
影响因子：
2
通讯作者：
Wen H
中科院分区：
文献类型：
--
作者：
Morales AG;Stempinski ES;Xiao X;Patel A;Panna A;Olivier KN;McShane PJ;Robinson C;George AJ;Donahue DR;Chen P;Wen H
透射电子显微镜（TEM）提供了体积样品的亚纳米级细节。诸如病理组织标本的样本通常用金属元素染色以增强对比度，这使得它们对光学显微镜不透明。因此，通过切片找到样品内部的感兴趣区域可能是一个漫长的过程。我们描述了微CT侦察TEM，允许非侵入性识别块样品内的感兴趣区域，以指导切片步骤。在一项组织病理学研究中，使用分辨率为10 μm的台式微型CT扫描仪来确定锇染色的人鼻刮样本中粘膜斑块的位置。一旦定位了感兴趣的区域，将样品块切片以暴露该位置，然后进行超薄切片和TEM以纳米分辨率检查膜上皮细胞纤毛的内部结构。这种方法大大减少了搜索过程的时间和劳动力，从通常的20个切片的光学显微镜（LM）到3个切片，没有添加样品制备。
Transmission electron microscopy (TEM) provides sub-nanometer-scale details in volumetric samples. Samples such as pathology tissue specimens are often stained with a metal element to enhance contrast, which makes them opaque to optical microscopes. As a result, it can be a lengthy procedure to find the region of interest inside a sample through sectioning. We describe micro-CT scouting for TEM that allows non-invasive identification of regions of interest within a block sample to guide the sectioning step. In a tissue pathology study, a bench-top micro-CT scanner with 10 μm resolution was used to determine the location of patches of the mucous membrane in osmium-stained human nasal scraping samples. Once the regions of interest were located, the sample block was sectioned to expose that location, followed by ultra-thin sectioning and TEM to inspect the internal structure of the cilia of the membrane epithelial cells with nanometer resolution. This method substantially reduced the time and labor of the search process from typically 20 sections for light microscopy (LM) to 3 sections with no added sample preparation.