Histopathologic and molecular alterations in bronchial epithelium in habitual smokers of marijuana, cocaine, and/or tobacco
Histopathologic and molecular alterations in bronchial epithelium in habitual smokers of marijuana, cocaine, and/or tobacco
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DOI：
10.1093/jnci/90.16.1198
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发表时间：
1998-08-19
影响因子：
10.3
通讯作者：
Tashkin, DP
中科院分区：
文献类型：
--
作者：
Barsky, SH;Roth, MD;Tashkin, DP
背景：吸烟已被观察到引起支气管上皮的分子改变，这种改变早于肺癌的发生。在美国年轻人中大麻和可卡因使用的流行率不断上升，促使我们调查是否类似的分子和组织病理学改变发生在大麻和/或可卡因的习惯吸烟者中，他们可能也吸烟，也可能不吸烟。对104名健康志愿者进行了支气管镜检查，其中包括28名不吸烟者和76名吸食大麻、烟草和/或可卡因一种或多种物质的吸烟者。分析支气管粘膜活检标本和刷牙的组织病理学变化，以及与癌症风险增加相关的中间或替代终点标志物的免疫组织病理学表达（Ki-67[细胞增殖标志物]，表皮生长因子受体，p53， Her-2/neu[也称为erbB-2和ERBB2]，球肌动蛋白和异常DNA倍体）。报告的P值是双面的。结果：吸烟任何一种物质或两种或两种以上物质的吸烟者比不吸烟者在5到9项所调查的PO组织病理学参数中表现出更多的改变
Background: Tobacco smoking has been observed to cause molecular alterations in bronchial epithelium that antedate the development of lung carcinoma, The rising prevalence of marijuana and cocaine use among young adults in the United States prompted us to investigate whether similar molecular and histopathologic alterations occur in habitual smokers of marijuana and/or cocaine who may or may not also smoke tobacco, Methods: Bronchoscopy was performed in 104 healthy volunteer subjects, including 28 nonsmokers and 76 smokers of one or more of the following substances: marijuana, tobacco, and/or cocaine. Bronchial mucosa biopsy specimens and brushings were analyzed for histopathologic changes, for immunohistopathologic expression of intermediate or surrogate end-point markers that are linked to an increased risk of cancer (Ki-67 [a marker of cell proliferations], epidermal growth factor receptor, p53, Her-2/neu [also known as erbB-2 and ERBB2], globular actin, and abnormal DNA ploidy). Reported P values are two-sided. Results: Smokers of any one substance or of two or more substances exhibited more alterations than nonsmokers in five to nine of the PO histopathologic parameters investigated (all P