Expression and function of conserved nuclear receptor genes in Caenorhabditis elegans
Expression and function of conserved nuclear receptor genes in Caenorhabditis elegans
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DOI：
10.1016/j.ydbio.2003.10.014
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发表时间：
2004-02-15
影响因子：
2.7
通讯作者：
Sluder, AE
中科院分区：
文献类型：
--
作者：
Gissendanner, CR;Crossgrove, K;Sluder, AE
秀丽线虫基因组编码284个核受体基因。在这284个NR基因中，有15个基因在后生动物中保守。在这里，我们分析了8个迄今尚未鉴定的线虫NR保守基因的结构、表达和功能。报告基因分析表明，这些基因有不同的表达模式，大多数线虫细胞类型表达保守的NR基因。RNA干扰NR基因的功能导致其中三个基因的表型可见，揭示了胚胎后发育的不同过程中的功能。其中5个保守的NR基因是在昆虫蜕皮和变态过程中起作用的NR基因的同源基因。功能研究证实了大多数‘蜕皮激素级联’的NR同源基因在持续生长和Dauer蜕皮过程中起到了作用。这些基因的转录水平在蜕皮周期中以重复的模式波动，这让人想起在昆虫蜕皮激素反应中观察到的表达层次。总之，这些分析为进一步剖析NRs在线虫发育中的作用以及评价后生动物中NR功能的保护提供了基础。(C)2003 Elsevier Inc.保留所有权利。
The Caenorhabditis elegans genome encodes 284 nuclear receptor (NR) genes. Among these 284 NR genes are 15 genes conserved among the Metazoa. Here, we analyze the, expression and function of eight heretofore uncharacterized conserved C elegans NR genes. Reporter gene analysis demonstrates that these genes have distinct expression patterns and that a majority of the C elegans cell types express a conserved NR gene. RNA interference with NR gene function resulted in visible phenotypes for three of the genes, revealing functions in various processes during postembryonic development. Five of the conserved NR genes are orthologs of NR genes that function during molting and metamorphosis in insects. Functional studies confirm a role for most of these 'ecdysone cascade' NR orthologs during the continuous growth and dauer molts. Transcript levels for these genes fluctuate in a reiterated pattern during the molting cycles, reminiscent of the expression hierarchy observed in the insect ecdysone response. Together, these analyses provide a foundation for further dissecting the role of NRs in nematode development as well as for evaluating conservation of NR functions among the Metazoa. (C) 2003 Elsevier Inc. All rights reserved.