Gene targeting by homologous recombination in Drosophila
Gene targeting by homologous recombination in Drosophila
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DOI：
10.1126/science.288.5473.2013
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发表时间：
2000-06-16
期刊：
影响因子：
56.9
通讯作者：
Golic, KG
中科院分区：
文献类型：
--
作者：
Rong, YS;Golic, KG
果蝇作为一种实验生物有许多优点。然而，与酵母和小鼠这两种广泛使用的真核模型系统相比，果蝇不能在引入的DNA和相应的染色体位点之间进行同源重组。特异性修饰酵母和小鼠基因组的能力提供了一种快速简便的方法来产生或拯救基因突变，其中DNA克隆或序列是可用的。描述了一种能够对果蝇基因组进行类似操作的方法。这种技术也可能适用于基因靶向程序尚不存在的其他生物体。
Drosophila offers many advantages as an experimental organism. However, in comparison with yeast and mouse, two other widely used eukaryotic model systems, Drosophila suffers from an inability to perform homologous recombination between introduced DNA and the corresponding chromosomal loci. The ability to specifically modify the genomes of yeast and mouse provides a quick and easy way to generate or rescue mutations in genes for which a DNA clone or sequence is available. A method is described that enables analogous manipulations of the Drosophila genome. This technique may also be applicable to other organisms for which gene-targeting procedures do not yet exist.