Determination of the duration of a primary immune response and the ID50 of ALA rabbit rotavirus in rabbits.
Determination of the duration of a primary immune response and the ID50 of ALA rabbit rotavirus in rabbits.
复制标题
测定兔体内 ALA 兔轮状病毒的初次免疫反应持续时间和 ID50。
DOI：
10.1007/s007050050242
复制
发表时间：
1997
影响因子：
2.7
通讯作者：
Estes,MK
中科院分区：
文献类型：
--
作者：
Conner,ME;Graham,DY;Estes,MK
轮状病毒感染的兔模型已用于检查对轮状病毒感染的免疫反应并评估轮状病毒疫苗的开发策略。为了确定组织培养适应的 ALA 病毒的 50% 感染剂量 (ID50)，兔子口服接种了 101-103 PFU 的 ALA 轮状病毒。测定ALA病毒的ID50为1.7×102PFU。在低病毒剂量（102-103PFU）感染的兔子中诱导的免疫反应与高剂量（106PFU）接种物诱导的免疫反应的程度相似，表明兔子对ALA轮状病毒的免疫反应不是剂量依赖性的。为了确定单次轮状病毒接种是否会诱导持久的免疫反应，四只兔子接种一次 ALA 病毒 (3.5 × 105PFU)，并每隔 1.5-2 年监测它们的血清学和粘膜抗轮状病毒滴度。受感染的兔子一直保持血清学和粘膜轮状病毒抗体滴度，直到接种后700多天的最终时间点。这些数据很重要，因为它们表明单次口服接种后获得的抗原负荷足以实现持久的免疫力，这是任何潜在疫苗的目标。
The rabbit model of rotavirus infection has been used to examine the immune response to rotavirus infection and to evaluate strategies for rotavirus vaccine development. To determine the 50% infectious does (ID50) of tissue culture adapted ALA virus, rabbits were orally inoculated with 101–103PFU of ALA rotavirus. The ID50of ALA virus was determined to be 1.7×102PFU. The immune response induced in rabbits infected at low virus doses (102–103PFU) was of similar magnitude to the immune responses induced with a high dose (106PFU) inoculum, indicating that the immune response to ALA rotavirus in rabbits is not dose dependent. To determine if a single rotavirus inoculation would induce a long lasting immune response, four rabbits were inoculated once with ALA virus (3.5 × 105PFU) and their serologic and mucosal antirotavirus titers were monitored at intervals for 1.5–2 years. The infected rabbits maintained serologic and mucosal rotavirus antibody titers until the final time point more than 700 days post inoculation. These data are important because they indicate that the antigenic load achieved following a single oral inoculation is sufficient to achieve long lasting immunity, the goal of any potential vaccine.