Anthelmintic activity in vitro of Citrus sinensis and Melaleuca quinquenervia essential oil from Cuba on Haemonchus contortus
Anthelmintic activity in vitro of Citrus sinensis and Melaleuca quinquenervia essential oil from Cuba on Haemonchus contortus
复制标题
DOI：
10.1016/j.indcrop.2015.07.056
复制
发表时间：
2015-12-15
影响因子：
5.9
通讯作者：
de Souza Chagas, Ana Carolina
中科院分区：
文献类型：
--
作者：
Aleman Gainza, Yousmel;Domingues, Luciana Ferreira;de Souza Chagas, Ana Carolina
胃肠道线虫是小型反刍动物生产中的一个重要问题，其控制几乎完全依赖于商业驱虫药。然而，寄生虫对这些化学制剂的抗性是一个日益严重的全球性问题，因此正在研究新的植物源化合物，以了解其对抗胃肠道线虫的潜在用途。通过卵孵化试验 (EHT) 和幼虫发育试验 (LDT)，分别在浓度范围为 0.02 mg/mL 至 50 mg/mL 和 0.04 mg/mL 至 3.12 mg/mL 的情况下，评估柑橘和五叶白千层精油对捻转血矛线虫 Embrapa2010 抗性分离株的效果。所有浓度、阳性对照（EHT 中噻菌灵或 LDT 中伊维菌素）和阴性对照（EHT 中 2% Tween 80 或 LDT 中 0.5% DMSO）均进行六次重复和三个独立实验，总共 18 次重复，每次处理涉及约 1800 只寄生虫。显着差异（P
Gastrointestinal nematodes are an important problem in the small ruminant production and their control nearly exclusively depends on commercial anthelmintics. However, parasite resistance to those chemical formulations is an increasing global problem, so new plant-derived compounds are being studied for their potential use against gastrointestinal nematodes. Citrus sinensis and Melaleuca quinquenervia essential oils were evaluated against Haemonchus contortus Embrapa2010 resistant isolate, through the egg hatch test (EHT) and larval development test (LDT) at concentrations ranging from 0.02 mg/mL to 50 mg/mL and from 0.04 mg/mL to 3.12 mg/mL, respectively. All concentrations, positive controls (thiabendazole in EHT or ivermectin in LDT), and negative controls (2% Tween 80 in EHT or 0.5% DMSO in LDT) were performed in six replicates and in three independent experiments, for a total of 18 repetitions involving approximately 1800 parasites per treatment. Significant differences (P