Expression of AODEF, a B-functional MADS-box gene, in stamens and inner tepals of the dioecious species Asparagus officinalis L.
Expression of AODEF, a B-functional MADS-box gene, in stamens and inner tepals of the dioecious species Asparagus officinalis L.
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DOI：
10.1023/a:1023097202885
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发表时间：
2003-04-01
影响因子：
5.1
通讯作者：
Kameya, T
中科院分区：
文献类型：
--
作者：
Park, JH;Ishikawa, Y;Kameya, T
园林芦笋（asparagus officinalis L.）是雌雄异株种，雄花和雌花在单独的单性个体上。由于具有B和c功能的MADS-box基因指定雄性和雌性生殖器官，因此对这些基因进行表征对于阐明雌雄异株物种的性别决定机制具有重要意义。本研究分离并鉴定了officinalis雄花和雌花发育中的B-功能基因AODEF基因。南方杂交在芦笋基因组中鉴定出AODEF基因的单拷贝。Northern blot分析表明，该基因在花蕾中特异性表达，而在营养组织中不表达。原位杂交表明，在雌雄同体早期，AODEF基因在雄花和雌花的内花被片和雄蕊轮（分别为2轮和3轮）中表达，而在外花被片（第1轮）中不表达。在单性发育后期，AODEF基因在雄花的内花被片和雄蕊中仍有表达，而在雌花的第2轮和第3轮中表达减少。结果表明，AODEF基因可能不参与芦笋花被的发育，在发育后期，AODEF基因的表达可能直接或间接受到性别决定基因的控制。
Garden asparagus (Asparagus officinalis L.) is a dioecious species with male and female flowers on separate unisexual individuals. Since B- and C-functional MADS-box genes specify male and female reproductive organs, it is important to characterize these genes to clarify the mechanism of sex determination in monoecious and dioecious species. In this study, we isolated and characterized AODEF gene, a B- functional gene in the development of male and female flowers of A. officinalis. Southern hybridization identified a single copy of AODEF gene in asparagus genome. Northern blot analysis showed that this gene was specifically expressed in flower buds and not in vegetative tissues. In situ hybridization showed that during early hermaphrodite stages, AODEF gene was expressed in the inner tepal and stamen whorls (whorls 2 and 3, respectively), but not in the outer tepals (whorl 1), in both male and female flowers. In late unisexual developmental stages, the expression of AODEF gene was still detected in the inner tepals and stamens of male flowers, but the expression was reduced in whorls 2 and 3 of female flowers. Our results suggest that AODEF gene is probably not involved in tepal development in asparagus and that the expression of AODEF gene is probably controlled directly or indirectly by sex determination gene in the late developmental stages.