The structure of the major cell wall polysaccharide of Bacillus anthracis is species-specific
The structure of the major cell wall polysaccharide of Bacillus anthracis is species-specific
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DOI：
10.1074/jbc.m605768200
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发表时间：
2006-09-22
影响因子：
4.8
通讯作者：
Carlson, Russell W.
中科院分区：
文献类型：
--
作者：
Choudhury, Biswa;Leoff, Christine;Carlson, Russell W.
在这份报告中，我们描述了从炭疽芽孢杆菌营养细胞壁释放的多糖的结构的含水氟化氢（HF）。从B的艾姆斯、Sterne和巴斯德菌株中分离出这种释放HF的多糖（HF-PS），并对其结构进行了表征。炭疽病HF-PS还从密切相关的蜡样芽孢杆菌ATCC 10987菌株和B中分离。蜡状芽孢杆菌ATCC 14579型菌株的产酶活性，并与B.炭疽病B的结构。通过糖基组成和连接分析、基质辅助激光解吸-飞行时间质谱以及一维和二维核磁共振光谱确定炭疽HF-PS。所有B的HF-PS。炭疽菌分离物具有相同的结构，由α-GlcNAc-（1 - 4）-β-ManNAc-（1 - 4）-β-GlcNAc-（1 - 4）的氨基糖骨架组成，其中α-GlcNAc残基在O-3和O-4处分别被α-Gal和β-Gal取代，β-GlcNAc在O-3处被α-Gal取代。在这三个Gal置换中的两个的存在下存在一些可变性。与来自B的HF-PS的比较。蜡状芽孢杆菌ATCC 10987和B.蜡状芽孢杆菌ATCC 14579菌株中，B.炭疽菌的结构明显不同于这些HF-PS中的每一个，此外，B.蜡状芽孢杆菌ATCC 10987 HF-PS结构与B不同。蜡状芽孢杆菌ATCC 14579。B的存在。讨论了其营养细胞壁中炭疽-顺式特异性多糖结构与其它芽孢杆菌属的关系。
In this report we describe the structure of the polysaccharide released from Bacillus anthracis vegetative cell walls by aqueous hydrogen fluoride (HF). This HF-released polysaccharide (HF-PS) was isolated and structurally characterized from the Ames, Sterne, and Pasteur strains of B. anthracis. The HF-PSs were also isolated from the closely related Bacillus cereus ATCC 10987 strain, and from the B. cereus ATCC 14579 type strain and compared with those of B. anthracis. The structure of the B. anthracis HF-PS was determined by glycosyl composition and linkage analyses, matrix-assisted laser desorption-time of flight mass spectrometry, and one- and two-dimensional nuclear magnetic resonance spectroscopy. The HF-PSs from all of the B. anthracis isolates had an identical structure consisting of an amino sugar backbone of -> 6)-alpha-GlcNAc-(1 -> 4)-beta-ManNAc-(1 -> 4)-beta-GlcNAc-(1 ->, in which the alpha-GlcNAc residue is sub-stituted with alpha-Gal and beta-Gal at O-3 and O-4, respectively, and the beta-GlcNAc substituted with alpha-Gal at O-3. There is some variability in the presence of two of these three Gal substitutions. Comparison with the HF-PSs from B. cereus ATCC 10987 and B. cereus ATCC 14579 showed that the B. anthracis structure was clearly different from each of these HF-PSs and, furthermore, that the B. cereus ATCC 10987 HF-PS structure was different from that of B. cereus ATCC 14579. The presence of a B. anthra-cis-specific polysaccharide structure in its vegetative cell wall is discussed with regard to its relationship to those of other Bacillus species.