Subcellular distribution and chemical form of Cd and Cd-Zn interaction in different barley genotypes
Subcellular distribution and chemical form of Cd and Cd-Zn interaction in different barley genotypes
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DOI：
10.1016/j.chemosphere.2005.01.071
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发表时间：
2005-09-01
期刊：
影响因子：
8.8
通讯作者：
Zhang, GP
中科院分区：
文献类型：
--
作者：
Wu, FB;Dong, J;Zhang, GP
通过水培试验，研究了4个大麦品种对Cd、Zn吸收的亚细胞分布、化学形态及其互作效应的遗传差异。基质中Cd浓度的增加导致根/地上部各部位Cd浓度显著增加，其中以FI(细胞壁)和FIV(可溶性)积累最多。在根中，2%HAc或0.6M HCl提取液中Cd含量最高，其次是1M NaC l(20%)，80%乙醇或d-H2O提取液中Cd含量最低。在地上部，1M氯化钠和2%HAc提取组分的Cd积累量最高，其次是0.6M HCl和d-H2O，而80%乙醇提取液的积累量最小。Cd含量在亚细胞形态和化学形态上存在显著差异，与其他3个抗性品种相比，Cd敏感品种乌毛岭的叶绿体/根(FII)、细胞器(FII)、细胞器(FII)、无机Cd和水溶性Cd含量较高，而FI、FIV和果胶/蛋白质结合Cd含量较低。Cd处理48h后，换入无Cd营养液中培养72h，根系Cd浓度显著下降，乌茅岭Cd减少幅度较小。与对照(不加Cd)相比，添加Cd显著增加了叶绿体(FII)中锌的积累，但乌茅岭的增加幅度最小。其余3种组分的锌含量随着Cd的添加而显著降低，尤其是根部，其中乌毛岭受影响最大。此外，补锌还能显著降低Cd处理植株根营养质体和地上部可溶性Cd的浓度。(C)2005年由爱思唯尔有限公司出版。
A hydroponic experiment was carried out to study the genotypic difference in subcellular distribution and chemical form of Cd and Zn uptake and their interaction in four barley genotypes. Increased Cd level in the medium caused a significant increase of Cd concentration in all fractions of roots/shoots, with most accumulation in FI (cell wall) and FIV (soluble). In root, the greatest amount of Cd was found in extraction solution of 2% HAC or 0.6 M HCl, followed by 1 M NaCl (20%), and lowest in extraction of 80% ethanol or d-H2O. While in shoot, the highest value of Cd accumulation was recorded in the fractions extracted by 1 M NaCl and 2% HAC, followed by 0.6 M HCl or d-H2O, and least in 80% ethanol extraction solution. There was a distinct difference among genotypes in Cd concentration in subcellular and chemical forms and it was found that the Cd-sensitive genotype Wumaoliuling, in comparison with the other three Cd-resistant genotypes, had higher Cd concentration in chloroplast-shoot/trophoplast-root (FII), membrane and organelle (FIII) and in inorganic and water-soluble Cd of roots, while lower in FI, FIV and pectates/protein integrated Cd. After 48 h of Cd treatment, the plants were replaced into Cd-free nutrient solution and grown for 72 h, a significant decrease in Cd concentration of root FI was observed, with less Cd reduction in Wumaoliuling. In comparison with control (no Cd), addition of Cd significantly increased Zn accumulation in chloroplast (FII), with least increase in Wumaoliuling. The Zn content in the other 3 fractions decreased significantly with addition of Cd, especially in roots, while Wumaoliuling was the most severely affected genotype. Moreover, it could be suggested Zn supplement could significantly reduce Cd concentration in root trophoplast and in shoot soluble fraction of Cd-treated plants. (C) 2005 Published by Elsevier Ltd.