Isolation and culture of bovine endothelial cells of endoneurial origin
Isolation and culture of bovine endothelial cells of endoneurial origin
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DOI：
10.1002/(sici)1097-4547(19970915)49:6
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发表时间：
1997-09-15
影响因子：
4.2
通讯作者：
Ikeda, K
中科院分区：
文献类型：
--
作者：
Kanda, T;Iwasaki, T;Ikeda, K
免疫球蛋白的渗透和/或活化的淋巴细胞向周围神经系统（PNS）实质的迁移是PNS免疫性疾病（包括格林-巴利综合征、IgM神经病和慢性炎性脱髓鞘性多神经根神经病）发生的最初关键步骤。因此，了解神经内膜组织来源的内皮细胞（endothelial cells of endothelial tissue origin，PnMEC）的细胞特性是很重要的，因为这些细胞构成了血-神经屏障（blood-nerve barrier，BNB）的主体。为此，我们建立了一种从牛马尾分离和培养纯PnMEC的方法，通过它们的鹅卵石外观、对因子VIII/血管性血友病因子（vWF）抗原的免疫反应性、DiI-Ac-LDL摄取阳性。这些细胞的葡萄糖转运蛋白1（GLUT 1）表达在体外迅速下调。除了作为主要鞘糖脂的GM 3（NeuAc）和GM 3（NeuGc）外，PnMEC还包括PNS实质共有的GM 1、GD 1a、GD 1b和GT 1b，以及作为次要种类的唾液酸乳糖胺基副糖甙（SLPG）。该方法将有助于PnMEC中表面分子的生化分析，所述表面分子可能在BNB的形成以及涉及PNS的病理状况中起关键作用。(C)1997 Wiley-Liss，Inc.
Penetration of immunoglobulins and/or migration of activated lymphocytes into peripheral nervous system (PNS) parenchyma are the initial key steps to develop immunological disorders of PNS including Guillain-Barre syndrome, IgM neuropathy and chronic inflammatory demyelinating polyradiculoneuropathy. Hence, it is important to know the cellular property of endothelial cells of endoneurial tissue origin (PnMEC) because these cells constitute the bulk of the blood-nerve barrier (BNB), For this purpose, we developed a method to isolate and culture pure populations of PnMECs from bovine cauda equina, PnMECs were identified by their cobblestone appearance, immunoreactivity against Factor VIII/von Willebrand factor (vWF) antigen, and positive uptake of DiI-Ac-LDL. The glucose transporter type 1 (GLUT1) expression of these cells was rapidly down-regulated in vitro, Other than GM3(NeuAc) and GM3(NeuGc) as major glycosphingolipids, PnMECs comprise GM1, GD1a, GD1b and GT1b, which are shared by PNS parenchyma, and sialyl lactosaminyl paragloboside (SLPG) as minor species, Because bovine PnMECs proliferate rapidly and a large mass of cells could be obtained, this method should contribute to the biochemical analysis of surface molecules in PnMECs that might play a key role in the formation of BNB as well as in pathological conditions involving the PNS. (C) 1997 Wiley-Liss, Inc.