Mitogen-induced liver hyperplasia does not substitute for compensatory regeneration during promotion of chemical hepatocarcinogenesis.
Mitogen-induced liver hyperplasia does not substitute for compensatory regeneration during promotion of chemical hepatocarcinogenesis.
复制标题
有丝分裂原诱导的肝脏增生并不能替代化学性肝癌发生过程中的代偿性再生。
DOI：
10.1093/carcin/13.3.379
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发表时间：
1992
期刊：
影响因子：
4.7
通讯作者：
Columbano,A
中科院分区：
文献类型：
--
作者：
Ledda-Columbano,GM;Coni,P;Curto,M;Giacomini,L;Faa,G;Sarma,DS;Columbano,A
实验旨在确定在暴露于几种肝肿瘤促进方案期间给予不同类型的肝细胞增殖刺激对酶改变肝细胞灶形成的功效。雄性Wistar大鼠注射二乙基亚硝胺（150 mg/kg体重）。恢复期2周后，分别给予促进方案、耐药肝细胞模型、苯巴比妥模型和山楂酸模型。当大鼠接受这些方案时，它们被给予肝细胞增殖刺激，要么是代偿型（三分之二部分肝切除术或致死性四氯化碳剂量），要么是直接增生刺激，如由主要有丝分裂原硝酸铅诱导的刺激。这些方案所促进的起始细胞被监测为γ-谷氨酰转移酶和胎盘谷胱甘肽s -转移酶阳性或腺苷三磷酸酶缺乏的酶改变肝细胞的焦点。虽然四氯化碳和部分肝切除术诱导的代偿性再生刺激了所使用方案的促进能力，但直接增生不能刺激起始肝细胞形成灶和/或结节。胸苷结合的评估表明，无论使用何种促进程序，在两种增殖刺激中DNA合成的程度没有显著差异。
Experiments were designed to determine the efficacy of different types of liver cell proliferative stimuli given during exposure to several liver tumor-promoting regimens, on the formation of foci of enzyme-altered hepatocytes. Male Wistar rats were initiated sith diethylnitrosamine (150 mg/kg body wt). After a 2 week recovery period animals were subjected to promoting regimens, the resistant hepatocyte model, the phenobarbital model and the orotic acid model. While the rats were on these regimens they were given liver cell proliferative stimulus, either a compensatory type (two-thirds partial hepatectomy or a necrogenic dose of carbon tetrachloride) or a direct hyperplastic stimulus such as that induced by the primary mitogen, lead nitrate. Initiated cells so promoted by these regimens were monitored as foci of enzyme-altered hepatocytes positive for γ-glutamyltransferase and placental glutathione S-transferase or deficient for adenosine triphosphatase. While carbon tetrachloride and partial hepatectomy-induced compensatory regeneration stimulated the promoting ability of the regimens used, direct hyperplasia could not stimulate the formation of foci and/or nodules from initiated hepatocytes. Evaluation of thymidine incorporation indicated that there was no significant difference in the extent of DNA synthesis in both the proliferative stimuli irrespective of the promoting procedure used.