A comparative genome-wide analysis of the ABC transporter gene family among three Gossypium species
A comparative genome-wide analysis of the ABC transporter gene family among three Gossypium species
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DOI：
10.1002/csc2.20525
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发表时间：
2021-06-11
期刊：
影响因子：
2.3
通讯作者：
Zhang, Yanjie
中科院分区：
文献类型：
--
作者：
Cui, Baolu;Li, Yan;Zhang, Yanjie
ATP结合盒（ABC）蛋白是一种功能强大的转运蛋白，参与植物发育的许多细胞过程。在本研究中，315，169和170 ABC基因在陆地棉，雷蒙棉和亚洲棉，分别确定。ABC基因的结构和系统发育特征的基础上，分为8个亚科。同一亚家族内的ABC成员具有相似的蛋白质结构和基序组成。串联和节段性重复事件似乎都塑造了ABC转运蛋白基因家族的广泛扩张，纯化选择是进化的主要驱动力。来自转录组数据的表达谱表现出不同的表达模式的GhABC基因在不同的组织和响应不同的非生物胁迫。此外，对10种组织中以及胚珠和纤维发育阶段的同线GhABC旁系同源物对的表达模式的分析揭示了它们在进化过程中的功能保守、亚功能化、新功能化和假基因化。最后，共表达网络和组织特异性表达谱的分析表明，几个ABC基因成员可能参与纤维发育。这项研究提供了一个全面的概述ABC转运蛋白基因家族，并形成进一步的功能分析在棉花的基础。
ATP-binding cassette (ABC) proteins are powerful transporters and are involved in countless cellular processes of plant development. In the present study, 315, 169, and 170 ABC genes were identified in Gossypium hirsutum, Gossypium raimondii, and Gossypium arboreum, respectively. The ABC genes were clustered into eight subfamilies based on their structural and phylogenetic features. The ABC members within the same subfamily showed similar protein structures and motif composition. Both tandem and segmental duplication events appear to have shaped the extensive expansion of the ABC transporter gene family, with purifying selection being the primary driving force for evolution. Expression profiles derived from transcriptome data exhibited distinct expression patterns of GhABC genes in various tissues and in response to different abiotic stresses. Additionally, analyses of the expression patterns of syntenic GhABC paralog pairs across 10 tissues and at ovule and fiber development stages revealed their functional conservation, subfunctionalization, neo-functionalization, and pseudogenization during evolution. Finally, the analysis of co-expressed subnetworks and tissue-specific expression profiles suggested several ABC gene members might be involved in fiber development. This research provides a comprehensive overview of the ABC transporter gene family and forms the basis for further functional analysis in cotton.