MICRODETERMINATION OF PROTEOGLYCANS AND GLYCOSAMINOGLYCANS IN THE PRESENCE OF GUANIDINE-HYDROCHLORIDE
MICRODETERMINATION OF PROTEOGLYCANS AND GLYCOSAMINOGLYCANS IN THE PRESENCE OF GUANIDINE-HYDROCHLORIDE
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DOI：
10.1016/0003-2697(87)90658-0
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发表时间：
1987-02-15
影响因子：
2.9
通讯作者：
HOFFMAN, HA
中科院分区：
文献类型：
--
作者：
CHANDRASEKHAR, S;ESTERMAN, MA;HOFFMAN, HA
该报告描述了一种微程序，可用于在用离液剂进行色谱洗脱后直接测量蛋白聚糖和糖胺聚糖。该测定基于硫酸化糖胺聚糖在溶液中与阳离子染料二甲基亚甲基蓝结合的能力。测定中加入氯化胍 (0.24 M) 可产生稳定的染料-蛋白聚糖相互作用，但消除了其他阴离子大分子（例如 DNA）的干扰。该测定快速、灵敏且可重复，因此可用于处理多个样品。最后，该程序可用于多种类型的蛋白聚糖和糖胺聚糖的定量测定。
This report describes a microprocedure that may be used for direct measurement of proteoglycans and glycosaminoglycans, after chromatographic elution with chaotropic reagents. The assay is based on the ability of the sulfated glycosaminoglycans to bind to the cationic dye, dimethylmethylene blue, in solution. Inclusion of guanidinium chloride (0.24 M) in the assay resulted in a stable dye-proteoglycan interaction, but eliminated the interference of other anionic macromolecules such as DNA. The assay is rapid, sensitive, and reproducible and therefore useful for processing several samples. Finally, the procedure can be used for quantitative determination of several types of proteoglycans and glycosaminoglycans.