Degradable polyester scaffolds with controlled surface chemistry combining minimal protein adsorption with specific bioactivation
Degradable polyester scaffolds with controlled surface chemistry combining minimal protein adsorption with specific bioactivation
复制标题
可降解聚酯支架具有受控表面化学，结合最小的蛋白质吸附和特定的生物活性
DOI：
10.1038/nmat2904
复制
发表时间：
2011-01-01
期刊：
影响因子：
41.2
通讯作者：
Groll, Juergen
中科院分区：
文献类型：
--
作者：
Grafahrend, Dirk;Heffels, Karl-Heinz;Groll, Juergen
用于组织工程的先进生物材料和支架对材料提出了很高的要求，并超过了以前认为生物医学植入物可接受的被动生物相容性要求（1-4）。与降解性一起，特定细胞-材料相互作用的激活和模拟细胞外基质的三维环境是活细胞组织为功能组织的核心挑战和先决条件（5）。此外，尽管生物活性信号传导与非特异性蛋白质吸附最小化相结合是一种用于平坦表面的先进改性技术（6），但通常不能用于组织工程中使用的三维纤维支架。在这里，我们提出了一个一步制备的完全合成的，生物活性和可降解的细胞外基质模拟支架通过静电纺丝，使用聚（D，L-丙交酯-共-乙交酯）作为基质聚合物。添加基于星形聚（环氧乙烷）的功能性两亲性大分子将当前生物医学上使用的可降解聚酯转化为亲水性纤维，这导致抑制纤维表面上的非特异性蛋白质吸附。细胞粘附介导肽随后共价连接到亲水性纤维促进特异性生物活化，并通过对固定化结合基序的排他性识别实现细胞粘附。这种方法允许合成材料直接控制细胞行为，例如，类似于细胞与固定在结缔组织的细胞外基质中的胶原纤维上的纤连蛋白的结合。
Advanced biomaterials and scaffolds for tissue engineering place high demands on materials and exceed the passive biocompatibility requirements previously considered acceptable for biomedical implants(1-4). Together with degradability, the activation of specific cell-material interactions and a three-dimensional environment that mimics the extracellular matrix are core challenges and prerequisites for the organization of living cells to functional tissue(5). Moreover, although bioactive signalling combined with minimization of non-specific protein adsorption is an advanced modification technique for flat surfaces(6), it is usually not accomplished for three-dimensional fibrous scaffolds used in tissue engineering. Here, we present a one-step preparation of fully synthetic, bioactive and degradable extracellular matrix-mimetic scaffolds by electrospinning, using poly(D,L-lactide-co-glycolide) as the matrix polymer. Addition of a functional, amphiphilic macromolecule based on star-shaped poly(ethylene oxide) transforms current biomedically used degradable polyesters into hydrophilic fibres, which causes the suppression of non-specific protein adsorption on the fibres' surface. The subsequent covalent attachment of cell-adhesion-mediating peptides to the hydrophilic fibres promotes specific bioactivation and enables adhesion of cells through exclusive recognition of the immobilized binding motifs. This approach permits synthetic materials to directly control cell behaviour, for example, resembling the binding of cells to fibronectin immobilized on collagen fibres in the extracellular matrix of connective tissue.