Age-related differences in human skin proteoglycans
Age-related differences in human skin proteoglycans
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DOI：
10.1093/glycob/cwq162
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发表时间：
2011-02-01
期刊：
影响因子：
4.3
通讯作者：
Caplan, Arnold I.
中科院分区：
文献类型：
--
作者：
Carrino, David A.;Calabro, Anthony;Caplan, Arnold I.
以前的研究表明，变态蛋白多糖、核心蛋白聚糖和一种被称为装饰蛋白的分解代谢片段，是人类皮肤中含量最丰富的蛋白多糖。对Verscan的进一步分析表明，从胎儿到成人，所有年龄的人皮肤中都存在四种主要的核心蛋白。其中两种被鉴定为V0和VI亚型，后者占主导地位。另两种为V0和VI的分解代谢片段，其羧基末端为氨基酸序列DPEAAE。虽然人类皮肤的核心蛋白没有明显的年龄差异，但成人皮肤的糖胺多聚糖(GAG)的大小较小，其硫酸盐化模式与胎儿皮肤的不同。与人类皮肤粘附素相比，人类皮肤装饰素在其硫酸盐化模式上显示出与年龄有关的最小差异，尽管像万西嘉人一样，成人皮肤装饰素的缝隙比胎儿皮肤装饰素的小。对成人皮肤中核心蛋白聚糖的分解代谢片段的分析表明，除了修饰外，还存在其他片段，尽管这些额外的核心蛋白尚未确定。因此，人类皮肤的粘附素和粘附素表现出年龄相关的差异，主要是粘附素的大小和硫酸盐化模式，以及粘附素的大小。凡西嘉的分解代谢片段在所有被检查的年龄段都被检测到，但与胎儿皮肤相比，成人皮肤中的分解代谢片段似乎较少。相反，粘附素的分解代谢片段在成人皮肤中存在，但在胎儿皮肤中几乎不存在。综上所述，这些数据表明，人类皮肤中蛋白多糖的分解代谢存在年龄相关的差异。这些与年龄相关的蛋白多糖模式和分解代谢的差异可能在人类皮肤物理特性和损伤反应的与年龄相关的变化中发挥作用。
Previous work has shown that versican, decorin and a catabolic fragment of decorin, termed decorunt, are the most abundant proteoglycans in human skin. Further analysis of versican indicates that four major core protein species are present in human skin at all ages examined from fetal to adult. Two of these are identified as the V0 and VI isoforms, with the latter predominating. The other two species are catabolic fragments of V0 and VI, which have the amino acid sequence DPEAAE as their carboxyl terminus. Although the core proteins of human skin versican show no major age-related differences, the glycosaminoglycans (GAGs) of adult skin versican are smaller in size and show differences in their sulfation pattern relative to those in fetal skin versican. In contrast to human skin versican, human skin decorin shows minimal age-related differences in its sulfation pattern, although, like versican, the GAGs of adult skin decorin are smaller than those of fetal skin decorin. Analysis of the catabolic fragments of decorin from adult skin reveals the presence of other fragments in addition to decorunt, although the core proteins of these additional decorin catabolic fragments have not been identified. Thus, versican and decorin of human skin show age-related differences, versican primarily in the size and the sulfation pattern of its GAGs and decorin in the size of its GAGs. The catabolic fragments of versican are detected at all ages examined, but appear to be in lower abundance in adult skin compared with fetal skin. In contrast, the catabolic fragments of decorin are present in adult skin, but are virtually absent from fetal skin. Taken together, these data suggest that there are age-related differences in the catabolism of proteoglycans in human skin. These age-related differences in proteoglycan patterns and catabolism may play a role in the age-related changes in the physical properties and injury response of human skin.