Treatment of murine peritoneal macrophages with bacterial lipopolysaccharide alters expression of c-fos and c-myc oncogenes.
Treatment of murine peritoneal macrophages with bacterial lipopolysaccharide alters expression of c-fos and c-myc oncogenes.
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DOI：
10.4049/jimmunol.137.8.2711
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发表时间：
1986-10
影响因子：
4.4
通讯作者：
M. Introna;T. Hamilton;R. Kaufman;D. Adams;R. Bast
中科院分区：
文献类型：
--
作者：
M. Introna;T. Hamilton;R. Kaufman;D. Adams;R. Bast
研究了巯基乙酸盐诱导的小鼠腹腔巨噬细胞暴露于脂多糖（LPS）后c-fos、c-myc和c-fms原癌基因的表达。与LPS（20 ng/ml）孵育后，可以观察到c-fos和c-myc癌基因表达的瞬时和快速诱导，而c-fms的RNA水平不受影响。用脂A（LPS分子的活性部分）处理，使c-fos和c-myc表达增加到相当的程度。用佛波醇肉豆蔻酸酯处理后，观察到类似的c-fos和c-myc诱导，表明LPS对小鼠巨噬细胞的这种作用可能通过刺激蛋白激酶C介导。在类似的实验条件下，LPS处理巨噬细胞不会触发DNA合成。用LPS处理阻断用含有集落刺激因子的L细胞条件培养基处理的巨噬细胞中的DNA合成。因此，c-fos和c-myc表达的变化可能是导致巨噬细胞活化的复杂系列生化事件中的元素，并且不一定与细胞增殖的诱导或引发相关。
Expression of the c-fos, c-myc, and c-fms proto-oncogenes has been studied in thioglycollate-elicited murine peritoneal macrophages after exposure to lipopolysaccharide (LPS). After incubation with LPS (20 ng/ml), a transient and rapid induction of the expression of c-fos and c-myc oncogenes could be observed, whereas the RNA levels for c-fms were not affected. Treatment with lipid A, the active moiety of the LPS molecule, increased the c-fos and c-myc expression to a comparable degree. Similar induction of c-fos and c-myc was observed after treatment with phorbol myristate acetate, suggesting that this effect of LPS on murine macrophages might be mediated through stimulation of protein kinase C. Under similar experimental conditions, LPS treatment of macrophages did not trigger DNA synthesis. Treatment with LPS blocked DNA synthesis in macrophages treated with L cell-conditioned medium containing colony-stimulating factor. Thus changes in c-fos and c-myc expression may be elements in the complex series of biochemical events that contribute to macrophage activation and are not necessarily related to induction or priming for cellular proliferation.