Creatine and nucleoside triphosphates in rat cerebral gray and white matter.
Creatine and nucleoside triphosphates in rat cerebral gray and white matter.
复制标题
大鼠大脑灰质和白质中的肌酸和三磷酸核苷。
DOI：
10.1007/bf02109364
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发表时间：
1995
影响因子：
3.6
通讯作者：
Holtzman,D
中科院分区：
文献类型：
--
作者：
Whittingham,TS;Douglas,A;Holtzman,D
内旋核磁共振波谱显示，与灰质相比，脑白质中磷酸肌酸/三磷酸核苷（PCr/NTP）的比率高出三倍。为了解释这些结果，在快速冷冻的大鼠大脑皮层和胼胝体样品中，用酶法测定了总肌酸（Cr）和ATP浓度。两个区域的总Cr （PCr + Cr）和ATP浓度相同。高效液相色谱分析显示，两个地区的NTP总浓度相似。这些结果表明，与灰质相比，白质中较高的活体opcr /NTP比率可能是由于白质中较高的PCr/Cr比率和/或灰质中较高的非腺嘌呤单核苷酸或二磷酸核苷酸百分比。
In vivolocalized nuclear magnetic resonance spectroscopy has shown three-fold higher phosphocreatine/nucleoside triphosphate (PCr/NTP) ratios in cerebral white compared to gray matter. To interpret these results, total creatine (Cr) and ATP concentrations were measured enzymatically in samples taken from rapidly frozen rat cerebral cortex and corpus callosum. Total Cr (PCr plus Cr) and ATP concentrations were the same in the two regions. High performance liquid chromatography showed similar concentrations of total NTP in the two regions. These results suggest that higherin vivoPCr/NTP ratios in white compared to gray matter may be due to a higher PCr/Cr ratio in white matter and/or higher percentages of non-adenine mono- or diphosphate nucleotides in gray matter.