Soluble TNFα receptor 1 and IL-6 plasma levels in humans subjected to the sleep deprivation model of spaceflight
Soluble TNFα receptor 1 and IL-6 plasma levels in humans subjected to the sleep deprivation model of spaceflight
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DOI：
10.1067/mai.2001.112270
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发表时间：
2001-01-01
影响因子：
14.2
通讯作者：
Dinges, DF
中科院分区：
文献类型：
--
作者：
Shearer, WT;Reuben, JM;Dinges, DF
背景资料：睡眠不足可能使宇航员在长时间太空旅行期间易患免疫力改变的程度促使地面模型进行评估。目的：我们试图测量选定的细胞因子及其受体的血浆水平，包括假定的睡眠调节蛋白可溶性TNF-α受体（sTNF-alphaR）I和IL-6，在具有性能要求的环境禁闭期间经历2种类型的睡眠剥夺的人类受试者中，方法：健康成年男性（n = 42）被随机分配到不同的睡眠丧失严重程度的时间表：4天（88小时）的部分睡眠剥夺（PSD），包括每天两次2小时的小睡或4天的完全睡眠剥夺（TSD）。在5天内每6小时采集一次血浆样本，并通过酶联免疫分析法检测sTNF-α RI、sTNF-α RII、IL-6、可溶性IL-2受体、IL-10和TNF-α。结果：时间和睡眠剥夺水平之间的相互作用检测到sTNF-α RI和IL-6，但未检测到sTNF-α RII、可溶性IL-2受体、IL-10和TNF-α。相对于PSD条件，TSD条件下的受试者在第2天血浆sTNF-α RI水平升高（P = .04），第3天（P = 0.01），在睡眠丧失的第2至4天，（P = .01）和第4天IL-6水平升高结论：睡眠不足导致sTNF-α RI和IL-6的血浆水平显著升高，它们是连接神经、内分泌和免疫系统的信使。这些变化似乎反映了睡眠的稳态驱动力的升高，因为它们发生在TSD而不是PSD，这表明午睡可能是长时间太空飞行的对策方法的基础。
Background: The extent to which sleep loss may predispose astronauts to a state of altered immunity during extended space travel prompts evaluation with ground-based models.Objective: we sought to measure plasma levels of selected cytokines and their receptors, including the putative sleep-regulation proteins soluble TNF-alpha receptor (sTNF-alphaR) I and IL-6, in human subjects undergoing 2 types of sleep deprivation during environmental confinement with performance demands,Methods: Healthy adult men (n = 42) were randomized to schedules that varied In severity of sleep loss: 4 days (88 hours) of partial sleep deprivation (PSD) involving two 2-hour naps per day or 4 days of total sleep deprivation (TSD). Plasma samples were obtained every 6 hours across 5 days and analyzed by using enzyme-linked immunoassays for sTNF-alpha RI, sTNF-alpha RII, IL-6, soluble IL-2 receptor, IL-10, and TNF-alpha.Results: Interactions between the effects of time and sleep deprivation level were detected for sTNF-alpha RI and IL-6 but not for sTNF-alpha RII, soluble IL-2 receptor, IL-10, and TNF-alpha. Relative to the PSD condition, subjects in the TSD condition had elevated plasma levels of sTNF-alpha RI on day 2 (P = .04), day 3 (P = .01), and across days 2 to 4 of sleep loss (P = .01) and elevated levels of IL-6 on day 4 (P = .04).Conclusions: Total sleep loss produced significant increases in plasma levels of sTNF-alpha RI and IL-6, messengers that connect the nervous, endocrine, and immune systems. These changes appeared to reflect elevations of the homeostatic drive for sleep because they occurred in TSD but not PSD, suggesting that naps may serve as the basis for a countermeasures approach to prolonged spaceflight.