Efficacy of Fludora® Fusion (a mixture of deltamethrin and clothianidin) for indoor residual spraying against pyrethroid-resistant malaria vectors: laboratory and experimental hut evaluation
Efficacy of Fludora® Fusion (a mixture of deltamethrin and clothianidin) for indoor residual spraying against pyrethroid-resistant malaria vectors: laboratory and experimental hut evaluation
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DOI：
10.1186/s13071-020-04341-6
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发表时间：
2020-09-11
影响因子：
3.2
通讯作者：
Ngufor, Corine
中科院分区：
文献类型：
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作者：
Fongnikin, Augustin;Houeto, Nadia;Ngufor, Corine
背景：新一代 IRS 杀虫剂正在开发中，它可以改善和延长对拟除虫菊酯耐药性疟疾病媒种群的控制。 Fludora (R) Fusion 是一种新型 IRS 杀虫剂，含有溴氰菊酯和噻虫胺（一种新烟碱类杀虫剂）的混合物。 方法：在贝宁 Cove 的实验室生物测定和实验小屋中，对混凝土和泥浆基质上的 Fludora (R) Fusion IRS 的功效进行了 11-12 个月的评估，以对抗野生自由飞翔的抗拟除虫菊酯类冈比亚按蚊（广义上的冈比亚按蚊）。对混合物中的两种活性成分进行了比较；噻虫胺和溴氰菊酯，单独使用。还进行了 CDC 瓶生物测定，以研究野生载体群体对噻虫胺的耐药性。结果 Fludora (R) Fusion 导致敏感和拟除虫菊酯抗性 An 的实验室锥体生物测定死亡率 > 80%。 gambiae(s.l.) 在混凝土块基材上使用 7-9 个月，在泥块基材上使用 12 个月。在 CDC 瓶生物测定中，实验小屋现场的载体群体对噻虫胺完全敏感。野生自由飞行拟除虫菊酯抗性An的总体死亡率。在为期 11 个月的试验期间，使用溴氰菊酯进入实验小屋的冈比亚(s.l.) 感染率 < 15%，而使用 Fludora (R) Fusion (69-71%) 和单独使用噻虫胺 (72-78%) 时则显着更高。 Fludora (R) Fusion 最初的实验小屋死亡率较高（> 80%），8 个月后仅下降了 50%。单独使用 Fludora (R) Fusion 和噻虫胺时，易感蚊子的每月原位壁锥生物测定死亡率 > 80%，持续 9-12 个月。与单独使用噻虫胺相比，Fludora (R) Fusion 诱导的蚊子早期离开率显着较高（55-60% vs 37-38%，P < 0.05）。 结论：室内滞留喷洒 Fludora (R) Fusion 导致野生拟除虫菊酯抗性疟疾病媒死亡率高且长期持续 7-10 个月，主要是由于噻虫胺成分，以及由于拟除虫菊酯成分。 Fludora (R) Fusion 是对当前 IRS 杀虫剂产品组合的重要补充，具有显着减少拟除虫菊酯抗性蚊媒传播疟疾的潜力。
Background: A new generation of IRS insecticides which can provide improved and prolonged control of pyrethroid-resistant malaria vector populations are being developed. Fludora (R) Fusion is a new IRS insecticide containing a mixture of deltamethrin and clothianidin, a neonicotinoid.Methods: The efficacy of Fludora (R) Fusion IRS was evaluated over 11-12 months on concrete and mud substrates in laboratory bioassays and experimental huts against wild free-flying pyrethroid-resistantAnopheles gambiae(sensu lato) in Cove, Benin. A comparison was made with the two active ingredients of the mixture; clothianidin and deltamethrin, applied alone. CDC bottle bioassays were also performed to investigate resistance to clothianidin in the wild vector population. Results Fludora (R) Fusion induced > 80% laboratory cone bioassay mortality with both susceptible and pyrethroid-resistantAn. gambiae(s.l.) for 7-9 months on concrete block substrates and 12 months on mud block substrates. The vector population at the experimental hut site was fully susceptible to clothianidin in CDC bottle bioassays. Overall mortality rates of wild free-flying pyrethroid-resistantAn. gambiae(s.l.) entering the experimental huts during the 11-month trial were < 15% with deltamethrin and significantly higher with Fludora (R) Fusion (69-71%) and clothianidin alone (72-78%). Initial high experimental hut mortality rates with Fludora (R) Fusion (> 80%) only declined by 50% after 8 months. Monthlyin situwall cone bioassay mortality of susceptible mosquitoes was > 80% for 9-12 months with Fludora (R) Fusion and clothianidin alone. Fludora (R) Fusion induced significantly higher levels of early exiting of mosquitoes compared to clothianidin alone (55-60% vs 37-38%,P < 0.05).Conclusions: Indoor residual spraying with Fludora (R) Fusion induced high and prolonged mortality of wild pyrethroid-resistant malaria vectors for 7-10 months mostly due to the clothianidin component and substantial early exiting of mosquitoes from treated huts due to the pyrethroid component. Fludora (R) Fusion is an important addition to the current portfolio of IRS insecticides with the potential to significantly reduce transmission of malaria by pyrethroid-resistant mosquito vectors.