Arabidopsis thaliana responds to colonisation of Piriformospora indica by secretion of symbiosis-specific proteins
Arabidopsis thaliana responds to colonisation of Piriformospora indica by secretion of symbiosis-specific proteins
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DOI：
10.1371/journal.pone.0209658
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发表时间：
2018-12
期刊：
影响因子：
3.7
通讯作者：
Johannes Thürich;Doreen Meichsner;A. Furch;J. Pfalz;Thomas Krüger;O. Kniemeyer;A. Brakhage;R. Oelmüller
中科院分区：
文献类型：
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作者：
Johannes Thürich;Doreen Meichsner;A. Furch;J. Pfalz;Thomas Krüger;O. Kniemeyer;A. Brakhage;R. Oelmüller
植物以互惠、寄生或中性的方式与多种真菌相互作用。形成的联系依赖于伙伴之间的营养物质和信号分子的交换。这包括一组参与防御、营养吸收或建立共生关系的不同蛋白质类别。在这里，我们分析了互惠共生的根内生真菌印度梨形孢子和拟南芥在单独培养或混合培养时的分泌组。有100多种蛋白质被鉴定为差异分泌蛋白质，包括与生长、发育、非生物和生物应激反应和粘液相关的蛋白质。虽然一些蛋白质以前被认为参与了植物-微生物的相互作用，但另一些蛋白质是在这种情况下新描述的。在共培养中发现的一种植物蛋白是PLAT1(多囊藻毒素、脂氧合酶、阿尔法毒素和三酰甘油脂肪酶)。PLAT1尚未与植物-真菌-互作相关，而是已知在非生物胁迫反应中发挥作用。在定植的根中，PLAT1以特定阶段的方式表现出基因表达的变化，而Plat1基因敲除的植物定植得更强。它与十字花科特有的内质网小体(ER小体)共定位，内质网小体参与防御化合物东参碱的形成。我们观察到受感染的拟南芥根中的内质网小体降解，以及东莨菪碱水平随着真菌的存在而发生变化。
Plants interact with a wide variety of fungi in a mutualistic, parasitic or neutral way. The associations formed depend on the exchange of nutrients and signalling molecules between the partners. This includes a diverse set of protein classes involved in defence, nutrient uptake or establishing a symbiotic relationship. Here, we have analysed the secretomes of the mutualistic, root-endophytic fungus Piriformospora indica and Arabidopsis thaliana when cultivated alone or in a co-culture. More than one hundred proteins were identified as differentially secreted, including proteins associated with growth, development, abiotic and biotic stress response and mucilage. While some of the proteins have been associated before to be involved in plant-microbial interaction, other proteins are newly described in this context. One plant protein found in the co-culture is PLAT1 (Polycystin, Lipoxygenase, Alpha-toxin and Triacylglycerol lipase). PLAT1 has not been associated with plant-fungal-interaction and is known to play a role in abiotic stress responses. In colonised roots PLAT1 shows an altered gene expression in a stage specific manner and plat1 knock-out plants are colonised stronger. It co-localises with Brassicaceae-specific endoplasmic reticulum bodies (ER-bodies) which are involved in the formation of the defence compound scopolin. We observed degraded ER-bodies in infected Arabidopsis roots and a change in the scopolin level in response to the presence of the fungus.