Chloroplast gene for Mr 32000 polypeptide of photosystem II in Euglena gracilis is interrupted by four introns with conserved boundary sequences.
Chloroplast gene for Mr 32000 polypeptide of photosystem II in Euglena gracilis is interrupted by four introns with conserved boundary sequences.
复制标题
细小裸藻光系统 II 的 Mr 32000 多肽的叶绿体基因被四个具有保守边界序列的内含子中断。
DOI：
10.1093/nar/12.14.5801
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发表时间：
1984
影响因子：
14.9
通讯作者：
Hallick,RB
中科院分区：
文献类型：
--
作者：
Karabin,GD;Farley,M;Hallick,RB
光系统II的Mr 32000除草剂结合多肽的基因先前已被定位到Euglena gralis叶绿体DNA的5 kbpEcoRI片段EcoRI上。测定了含有psbA基因座的EcoI的3324 bp核苷酸序列。该基因座编码345个氨基酸的多肽，其与来自四种高等植物叶绿体psbA基因座的序列共线性，并且具有86%的衍生氨基酸序列同源性。EuglenapsbA基因含有4个内含子，大小分别为435、443、434和617 bp。四个内含子具有先前在眼虫属叶绿体基因（rbcL）中描述的核酮糖-1，5-二磷酸羧化酶大亚基的类型的保守边界序列（Koller等人，36号牢房545-553 1984年）。
The gene for the Mr32000 herbicide binding polypeptide of photosystem II has previously been mapped to the 5 kbpEcoRI fragmentEcoI ofEuglena gralischloroplast DNA. The nucleotide sequence of 3324 bp ofEcoI, containing the psbA locus, has been determined. This locus encodes a polypeptide of 345 amino acids which is co-linear with, and has 86% derived amino acid sequence homology to sequences derived from four higher plants chloroplast psbA loci. TheEuglenapsbA gene contains four introns of size 435, 443, 434, and 617 bp. The four introns have conserved boundary sequences of the type previously described in the Euglena chloroplast gene (rbcL) for the large subunit of ribulose-l,5-bisphoaphate carboxylase (Koller et al., Cell36, 545–553. 1984).