THE ROLE OF RNA MOLECULES IN TRANSDUCTION OF THE PROTOONCOGENE C-FPS
THE ROLE OF RNA MOLECULES IN TRANSDUCTION OF THE PROTOONCOGENE C-FPS
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DOI：
10.1016/0092-8674(86)90016-4
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发表时间：
1986-03-28
期刊：
影响因子：
64.5
通讯作者：
BISHOP, JM
中科院分区：
文献类型：
--
作者：
HUANG, CC;HAY, N;BISHOP, JM
逆转录病毒转导细胞基因需要两次重组：一次重组形成细胞基因和病毒基因组之间的左侧连接，另一次重组形成右侧连接。先前的发现提出了右手重组可能使用RNA分子作为中间体的可能性。我们现在通过证明禽肉瘤病毒PRCII中v-fps的右端是由原癌基因c-fps的mRNA 3“端的poly（A）区重组形成的，为这一观点提供了直接的实验支持。这种复制可以通过逆转录过程中的“拷贝选择”来介导，作用于同源或非同源核苷酸序列。
Transduction of cellular genes by retroviruses requires two recombinations: one to form the left-hand junction between cellular gene and viral genome, the other to form the right-hand junction. Previous findings raised the possibility that the right-hand recombination might use RNA molecules as intermediates. We now provide direct experimental support for this view by showing that the right-hand end of v-fps in the avian sarcoma virus PRCII was formed by recombination within the poly(A) tract at the 3'' end of the mRNA for the proto-oncogene c-fps. Recombination of this sort may be mediated by "copy-choice" during reverse transcription, acting on either homologous or non-homologous nucleotide sequences.