Sleep-related c-Fos protein expression in the preoptic hypothalamus: effects of ambient warming
Sleep-related c-Fos protein expression in the preoptic hypothalamus: effects of ambient warming
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DOI：
10.1152/ajpregu.2000.279.6.r2079
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发表时间：
2000-12-01
影响因子：
2.8
通讯作者：
McGinty, D
中科院分区：
文献类型：
--
作者：
Gong, H;Szymusiak, R;McGinty, D
视前区（POA）神经元活动促进睡眠，但关键的睡眠活跃神经元的定位尚不完全清楚。 POA 的热刺激也有利于睡眠。本研究使用 c-Fos 蛋白免疫染色方法在对照（22 摄氏度）和轻度升高（31.5 摄氏度）环境温度下定位 POA 睡眠活跃神经元。在 22 摄氏度下，睡眠后而非醒来后，我们发现中视前核 (MnPN) 的头侧和尾侧以及腹外侧视前区 (VLPO) 的 c-Fos 免疫反应神经元 (IRN) 数量增加。在 31.5 摄氏度睡眠的动物中，与在 22 摄氏度睡眠的动物相比，在 MnPN 嘴侧发现了明显更多的 Fos IRN。在 VLPO 中，在升高的环境温度下睡眠后，Fos IRN 计数不再比清醒时的水平增加。 POA 中也广泛发现了与睡眠相关的 Fos IRN，但计数低于醒来后的计数。这项研究支持这样一种假设，即 MnPN 以及 VLPO 是 POA 睡眠促进系统的一部分，并且喙侧 MnPN 可能促进睡眠，特别是在环境温度升高的情况下。
Preoptic area (POA) neuronal activity promotes sleep, but the localization of critical sleep-active neurons is not completely known. Thermal stimulation of the POA also facilitates sleep. This study used the c-Fos protein immunostaining method to localize POA sleep-active neurons at control (22 degrees C) and mildly elevated (31.5 degrees C) ambient temperatures. At 22 degrees C, after sleep, but not after waking, we found increased numbers of c-Fos immunoreactive neurons (IRNs) in both rostral and caudal parts of the median preoptic nucleus (MnPN) and in the ventrolateral preoptic area (VLPO). In animals sleeping at 31.5 degrees C, significantly more Fos IRNs were found in the rostral MnPN compared with animals sleeping at 22 degrees C. In VLPO, Fos IRN counts were no longer increased over waking levels after sleep at the elevated ambient temperature. Sleep-associated Fos IRNs were also found diffusely in the POA, but counts were lower than those made after waking. This study supports a hypothesis that the MnPN, as well as the VLPO, is part of the POA sleep-facilitating system and that the rostral MnPN may facilitate sleep, particularly at elevated ambient temperatures.