SELECTION OF THE INITIATOR TRANSFER-RNA BY ESCHERICHIA-COLI INITIATION-FACTORS
SELECTION OF THE INITIATOR TRANSFER-RNA BY ESCHERICHIA-COLI INITIATION-FACTORS
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DOI：
10.1101/gad.3.12a.1899
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发表时间：
1989-12-01
影响因子：
10.5
通讯作者：
GOLD, L
中科院分区：
文献类型：
--
作者：
HARTZ, D;MCPHEETERS, DS;GOLD, L
我们已经开发了一种新技术，叫做“足印”，这使得研究大肠杆菌翻译起始复合物的trna结合特性成为可能。在天然mrna的作用下，只要在Shine和Dalgarno序列附近存在同源密码子，启动子tRNA和多种延长子tRNA就会结合到30S核糖体亚基上相同的tRNA结合位点。30S起始复合物中启动剂tRNA的选择是由起始因子IF2和IF3完成的。70S核糖体接受天然mrna上的启动体tRNA和延长体tRNA，很像30S核糖体亚基；然而，IF3和IF2不选择70S起始复合物上的起始tRNA，除非起始因子IF1存在。
We have developed a new technique, called ''toeprinting,'' which has allowed a study of the tRNA-binding properties of Escherichia coli translation initiation complexes. In response to natural mRNAs, the initiator tRNA and a variety of elongator tRNAs bind to the same tRNA-binding site on the 30S ribosomal subunit as long as a cognate codon is present near the Shine and Dalgarno sequence. The selection of the initiator tRNA in 30S initiation complexes is accomplished by initiation factors IF2 and IF3. 70S ribosomes accept both initiator tRNA and elongator tRNAs on natural mRNAs, much like 30S ribosomal subunits; IF3 and IF2 do not, however, select the initiator tRNA on 70S initiation complexes unless the initiation factor IF1 is present.