Cell surface labelling with gold colloid particulates: the use of avidin and staphylococcal protein A‐coated gold in conjunction with biotin and fc‐bearing ligands
Cell surface labelling with gold colloid particulates: the use of avidin and staphylococcal protein A‐coated gold in conjunction with biotin and fc‐bearing ligands
复制标题
用金胶体颗粒标记细胞表面：使用抗生物素蛋白和葡萄球菌蛋白 A 包被的金与生物素和带有 fc 的配体结合使用
DOI：
10.1111/j.1365-2818.1981.tb01296.x
复制
发表时间：
1981
影响因子：
2
通讯作者：
C. Hopkins
中科院分区：
文献类型：
--
作者：
N. Tolson;B. Boothroyd;C. Hopkins
描述了制备用亲和素或蛋白A稳定的金胶体颗粒的方法。概述了使用这些通用示踪剂定位生物素化和含fc免疫球蛋白的方法，并作为这些方法应用的示例，描述了鉴定卵巢颗粒细胞上表皮生长因子受体和表面纤维连接蛋白的程序。
Procedures for preparing gold colloid particles stabilized with either avidin or protein A are described. Methods of using these general utility tracers for localizing biotinylated and fc bearing immunoglobulins are outlined and, as examples of the way in which these methods can be applied, procedures for identifying epidermal growth factor receptors and surface fibronectin on ovarian granulosa cells are described.