Toward Full Peptide Sequence Coverage by Dual Fragmentation Combining Electron-Transfer and Higher-Energy Collision Dissociation Tandem Mass Spectrometry
Toward Full Peptide Sequence Coverage by Dual Fragmentation Combining Electron-Transfer and Higher-Energy Collision Dissociation Tandem Mass Spectrometry
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DOI：
10.1021/ac3025366
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发表时间：
2012-11-20
影响因子：
7.4
通讯作者：
Mohammed, Shabaz
中科院分区：
文献类型：
--
作者：
Frese, Christian K.;Altelaar, A. F. Maarten;Mohammed, Shabaz
通过串联质谱法增加肽序列覆盖率提高了基于数据库搜索的肽鉴定的置信度，并促进了翻译后修饰和从头测序的映射。通过结合电子转移和高能碰撞解离（EThcD）诱导2倍片段化产生双片段离子系列并促进广泛的肽骨架片段化。在初始电子转移解离步骤之后，包括未反应的前体离子的所有离子经受碰撞诱导解离，这在单个谱中产生B/y-和c/z-型碎片离子。这种新的片段化方案提供了更丰富的光谱，并大大增加了肽序列的覆盖率和肽鉴定的置信度。
Increasing peptide sequence coverage by tandem mass spectrometry improves confidence in database search-based peptide identification and facilitates mapping of post-translational modifications and de novo sequencing. Inducing 2-fold fragmentation by combining electron-transfer and higher-energy collision dissociation (EThcD) generates dual fragment ion series and facilitates extensive peptide backbone fragmentation. After an initial electron-transfer dissociation step, all ions including the unreacted precursor ions are subjected to collision induced dissociation which yields b/y- and c/z-type fragment ions in a single spectrum. This new fragmentation scheme provides richer spectra and substantially increases the peptide sequence coverage and confidence in peptide identification.