Phylogeny and historical demography of Cynops pyrrhogaster (Amphibia: Urodela): Taxonomic relationships and distributional changes associated with climatic oscillations
Phylogeny and historical demography of Cynops pyrrhogaster (Amphibia: Urodela): Taxonomic relationships and distributional changes associated with climatic oscillations
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DOI：
10.1016/j.ympev.2012.10.015
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发表时间：
2013-03-01
影响因子：
4.1
通讯作者：
Ota, Hidetoshi
中科院分区：
文献类型：
--
作者：
Tominaga, Atsushi;Matsui, Masafumi;Ota, Hidetoshi
我们利用线粒体DNA(NADH6、tRNAglu、cyt6)的1407个核苷酸序列和核DNA(RAG-1)的1208个核苷酸序列，研究了日本大陆火腹螈的系统发育关系并估计了其历史人口学特征。基于线粒体DNA的系统发育树揭示了该物种的四个主要单倍型分支(北部、中部、西部和南部分支)。种内变异的主要单倍型分支之间的遗传分化程度很大，表明该种由4个地理上相互替代的物种谱系组成。核遗传变异没有表现出明显的地理结构模式，只有线粒体单倍型的北方分支诊断的群体具有明显的区分性，这表明这是不完全谱系分类的结果。目前该属的分布和估计的分化时间表明，两个日本物种(琉球群岛的鲤鱼和尾鱼)的共同祖先在大陆边缘出现了分歧，相当于现在的东中国海和中部琉球。随后向日本大陆的范围扩张似乎发生在中新世中期。种群遗传分析表明，除南部物种外，所有物种谱系都经历了与冰川和冰后气候振荡有关的地理种群减少和扩张。(C)2012 Elsevier Inc.保留所有权利。
We investigated the phylogenetic relationships and estimated the historical demography of the Japanese fire-bellied newt, Cynops pyrrhogaster, from Japanese mainlands using 1407-bp sequences of the mitochondrial DNA (NADH6, tRNAglu, cyt 6) and 1208-bp sequences of nuclear DNA (Rag-1) genes. Phylogenetic trees based on mitochondrial DNA revealed four major haplotype clades (NORTHERN, CENTRAL, WESTERN, and SOUTHERN clades) within this species. Degree of genetic differentiation among major haplotype clades was very large for intraspecific variation, suggesting this species to be composed of four species lineages that replace each other geographically. Nuclear genetic variation presented no obvious patterns of geographic structure except for the distinctness of populations diagnosed by NORTHERN clade of mitochondrial haplotypes, suggesting results of incomplete lineage sorting. Current distribution and estimated divergence times for the genus Cynops suggest that the common ancestor of two Japanese species (C pyrrhogaster and C ensicauda from the Ryukyu Islands) had diverged at the edge of the continent corresponding to the present East China Sea and Central Ryukyus. Subsequent range expansion to Japanese mainland seems to have occurred in the middle Miocene. Population-genetic analyses indicated that all species lineages, except for the SOUTHERN one, experienced geographic population reductions and expansions associated with glacial and postglacial climatic oscillations. (C) 2012 Elsevier Inc. All rights reserved.