A comparison of levels of intrinsic single strand breaks/alkali labile sites associated with human melanoma cell invasion.
A comparison of levels of intrinsic single strand breaks/alkali labile sites associated with human melanoma cell invasion.
复制标题
与人类黑色素瘤细胞侵袭相关的内在单链断裂/碱不稳定位点水平的比较。
DOI：
10.1016/0304-3835(90)90009-m
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发表时间：
1990
期刊：
影响因子：
9.7
通讯作者：
Hendrix,MJ
中科院分区：
文献类型：
--
作者：
Meade-Tollin,LC;Pipes,BL;Anderson,SJ;Seftor,EA;Hendrix,MJ
已使用 DNA 过滤洗脱方法测定了不同体外侵袭能力的人黑色素瘤细胞群中无蛋白质单链断裂/碱不稳定位点的内在水平。对来自两种人类黑色素瘤细胞系 A375P 和 C8161 以及选自 A375P 的亚群 A375P-5 的 DNA 进行了分析，以检验 DNA 损伤水平增加可能与侵袭和转移能力增加的表型相关的假设。获得的洗脱曲线显示单链断裂和/或碱不稳定位点 (SSB ALS) 水平在统计上显着增加，这与侵袭和转移能力的增加相关。在新鲜选择的细胞中观察到的 A375P-5 中 SSB ALS 水平的增加随着这些细胞维持在培养物中而下降。这种 A375P-5 表型的稳定性与之前报道的双微小染色体水平相关，双微小染色体是基因组不稳定的指标。因此，细胞病变平均内在水平的改变是侵袭性和转移性肿瘤细胞表型特征中需要考虑的另一个因素，并且可能反映或促成肿瘤细胞群体的基因组不稳定性特征。
Intrinsic levels of protein-free single strand breaks/alkali-labile sites in human melanoma cell populations of varying in vitro invasive capacity have been assayed with DNA filter elution methodology. DNA from two human melanoma cell lines, A375P and C8161, and from a subpopulation selected from A375P, A375P-5, were assayed to test the hypothesis that increased levels of DNA damage may be associated with the phenotype of increased invasive and metastatic capacities. The elution profiles obtained reveal statistically significant increases in the level of single strand breaks and/or alkali-labile sites (SSB ALS) which correlate with increasing invasive and metastatic capacities. The increased levels of SSB ALS in A375P-5 observed in freshly selected cells decline as these cells are maintained in culture. The stability of this A375P-5 phenotype correlates with previously reported levels of double minute chromosomes, an indicator of genomic instability. Alterations in average intrinsic levels of cellular lesions are therefore an additional factor to be considered in the phenotypic characterization of invasive and metastatic tumor cells and may reflect or contribute to the genomic instability characteristic of tumor cell populations.